B046
Exploring microbial ecosystems using cutting edge advances in isotope and omics analyses

Session ID#: 75692

Session Description:
Microbial communities drive many biogeochemical processes which, as a result, impact nutrient availability, system productivity, and relevant fluxes from natural ecosystems. The complexity of these microbial communities, however, makes them challenging to study and can confound efforts to identify metabolic interactions between organisms, quantify gross and net metabolic fluxes, or reveal physiological interactions between plants, animals, and their geochemical environment.  Isotope and ‘omics’ analyses have historically provided tools for exploring these systems. Emerging advances linking isotope analysis to ‘omics’ approaches as well as improvements in measurement hardware are providing new insights to advance our scientific understanding of complex systems.  This session seeks to include discussions on linking isotope and ‘omic’ data collection, alternative isotope measurement platforms (i.e., spectroscopy and NMR), spatial isotope analysis, the use of multi- and non-traditional stable isotopes, and other related topics.  Both methodological and application-based presentations are encouraged.
Index Terms:

0414 Biogeochemical cycles, processes, and modeling [BIOGEOSCIENCES]
0448 Geomicrobiology [BIOGEOSCIENCES]
0454 Isotopic composition and chemistry [BIOGEOSCIENCES]
0465 Microbiology: ecology, physiology and genomics [BIOGEOSCIENCES]

Primary Convener:  James Moran, Pacific Northwest National Laboratory, Environmental Molecular Sciences Laboratory, Richland, WA, United States
Conveners:  Paul Dijkstra, Northern Arizona Univ, Flagstaff, AZ, United States and Steven Blazewicz, Lawrence Livermore National Laboratory, Livermore, CA, United States
Primary Liaison:  James Moran, Pacific Northwest National Laboratory, Environmental Molecular Sciences Laboratory, Richland, WA, United States
 
Improving nanoSIMS Quantification of Microbial Anabolic Activity: Constraining the Effect of Sample Preparation on Isotope Ratios (497716)
Nicolette Ruimin Meyer, Julian L Fortney and Anne E Dekas, Stanford University, Department of Earth System Science, Stanford, CA, United States
 
Interpreting lacustrine bulk sediment d15N values using metagenomics: A case study from a hypersaline lake system (514026)
Mingfei Chen1, Jessica L Conroy2, Robert A. Sanford3, Joanne Chin Chee-Sanford4 and Lynn M. Connor4, (1)University of Illinois at Urbana Champaign, Department of Geology, Urbana, IL, United States, (2)University of Illinois at Urbana Champaign, Urbana, IL, United States, (3)University of Illinois at Urbana-Champaign, Department of Geology, Urbana, IL, United States, (4)University of Illinois at Urbana Champaign, Department of Natural Resources and Environmental Sciences, Urbana, IL, United States
 
Improving Marker Gene Sequencing and Analysis Using Curated Single-Gene Databases: nifH as a Case Study (525238)
Bennett James Kapili and Anne E Dekas, Stanford University, Department of Earth System Science, Stanford, CA, United States
 
Nutrients and Heavy Metals Mediate the Distribution of Microbial Community in the Marine Sediments of the Bohai Sea, China (534330)
Meiqing Lu1,2, Xin Luo1, Jiu Jiu Jiao1, Hailong Li2, Xuejing Wang2, Jingyan Gao2, Xiaolang Zhang1 and Kai Xiao2, (1)The University of Hong Kong, Department of Earth Sciences, Hong Kong, China, (2)Southern University of Science and Technology, School of Environmental Science and Engineering, Shenzhen, China
 
Using 13C to Unravel the Molecular Mechanisms of Microbiome Response to Rewetting in Soil (546193)
Mary Suzanne Lipton1, Karl K Weitz2, Montana L Smith2, Sneha P Couvillion2, Vanessa L Paurus2, Thomas O Metz2, Emily Bonnell Graham3, Janet Jansson2 and Kirsten S Hofmockel2, (1)Pacific Northwest National Laboratory, Environmental and Molecular Sciences, Richland, WA, United States, (2)Pacific Northwest National Laboratory, Richland, WA, United States, (3)Pacific Northwest National Laboratory, Biological Sciences, Richland, WA, United States
 
Quantitative Stable Isotope Probing with Internal Standards. (553144)
Egbert Schwartz1, Michaela Hayer2, Bruce A Hungate1 and Katheryn Nantz3, (1)Northern Arizona University, Department of Biological Sciences and the Center for Ecosystem Science and Society, Flagstaff, AZ, United States, (2)Northern Arizona University, Center for Ecosystem Science and Society, Flagstaff, AZ, United States, (3)Northern Arizona University, Center for Ecosystem Science and Society, Biological Sciences, Flagstaff, AZ, United States
 
Evaluating the distribution and genomic content of novel microbes in deep-sea sediments (557486)
Alma Elizabeth Parada1, Julian L Fortney2, Alex Bishara3, Eli Moss3, Ami Bhatt3 and Anne E Dekas2, (1)Stanford University, Stanford, CA, United States, (2)Stanford University, Department of Earth System Science, Stanford, CA, United States, (3)Stanford University, Stanford, United States
 
Modeling Respiration of Individual Bacterial Populations in situ with Quantitative Stable Isotope Probing (qSIP) (558918)
Bram Stone1, Junhui Li2, Benjamin Koch1, Steven Blazewicz3, Paul Dijkstra4, Michaela Hayer5, Kirsten S Hofmockel6, Xiao-Jun Allen Liu7,8, Rebecca L Mau1,9, Ember Morrissey10, Jennifer Pett-Ridge3, Egbert Schwartz1 and Bruce A Hungate11, (1)Center for Ecosystem Science and Society, Flagstaff, AZ, United States, (2)Center for Ecosystem Science and Society, Flagstaff, United States, (3)Lawrence Livermore National Laboratory, Livermore, CA, United States, (4)Northern Arizona Univ, Flagstaff, AZ, United States, (5)Northern Arizona University, Center for Ecosystem Science and Society, Flagstaff, AZ, United States, (6)Pacific Northwest National Laboratory, Richland, WA, United States, (7)Northern Arizona University, Flagstaff, AZ, United States, (8)University of Massachusetts Amherst, Department of Microbiology, Amherst, MA, United States, (9)Pathogen Microbiome Institute, Flagstaff, AZ, United States, (10)West Virginia University, Division of Plant and Soil Sciences, Morgantown, WV, United States, (11)Northern Arizona University, Department of Biological Sciences and the Center for Ecosystem Science and Society, Flagstaff, AZ, United States
 
Insights into the biodegradation of sulfolane, an emerging groundwater contaminant, through stable isotope probing coupled to metagenomics (562225)
Mary Beth Leigh, University of Alaska Fairbanks, Institute of Arctic Biology, Fairbanks, AK, United States, Chris P Kasanke, Pacific Northwest National Laboratory, Richland, United States and Eric Collins, University of Alaska Fairbanks, Institute of Marine Science, Fairbanks, AK, United States
 
Stable Isotope Probing Reveals the Influence of Changing Redox Periodicity on Microbial Community Structure and Function in a Wet Tropical Forest Soil (578786)
Rachel Hestrin1, Ashley Campbell1, Jeff Kimbrel1, Amrita Bhattacharyya2, Gareth George Trubl1, Yang Lin3, Whendee L Silver3, Peter S Nico2, Steven Blazewicz1 and Jennifer Pett-Ridge1, (1)Lawrence Livermore National Laboratory, Livermore, CA, United States, (2)Lawrence Berkeley National Laboratory, Berkeley, CA, United States, (3)University of California Berkeley, Department of Environmental Science, Policy, and Management, Berkeley, CA, United States
 
Microbial Succession in Hypersaline, Alkaline Mono Lake, CA (585389)
Alexandra Atlee Phillips1, Daan R Speth1, Laurence G Miller2, Fenfang Wu1, Xingchen Tony Wang3, Danielle Potocek4, Patricia M Medeiros5, Magdalena R Osburn6, Hope Johnson7, Lizzy Trower8, William Berelson9, A. Joshua West10, Woodward W Fischer11, Victoria J Orphan1, Hannah Betts12, Alex L Sessions1 and Geobiology Course 2017 & 2018, (1)California Institute of Technology, Pasadena, CA, United States, (2)USGS, Menlo Park, CA, United States, (3)Princeton University, Princeton, NJ, United States, (4)California Institute of Technology, Pasadena, United States, (5)University of Georgia, Department of Marine Sciences, Athens, GA, United States, (6)Northwestern University, Earth and Planetary Sciences, Evanston, IL, United States, (7)California State University Fullerton, Fullerton, CA, United States, (8)Stanford University, Stanford, CA, United States, (9)University of Southern California, Los Angeles, CA, United States, (10)Univ. of Southern California, Los Angeles, CA, United States, (11)Caltech, Pasadena, CA, United States, (12)Westridge High School, Pasadena, United States
 
Tracing Root Exudate into the Rhizosphere Using Spatially Resolved Carbon Isotope Measurements (599347)
James Moran1, Timothy J Linley2, Jason Kriesel3, Elizabeth H Denis2, Peter Ilhardt2, Camille Makarem4, Jim Kelly5 and M. Lizabeth L Alexander1, (1)Pacific Northwest National Laboratory, Environmental Molecular Sciences Laboratory, Richland, WA, United States, (2)Pacific Northwest National Laboratory, Richland, WA, United States, (3)Opto-Knowledge Systems Inc, Torrance, CA, United States, (4)Opto-Knowledge Systems, Inc., Torrance, United States, (5)Louisville, KY, United States
 
Tracing C, N and S exchange in a nitrogen-fixing association (599534)
Rhona Stuart1, Eric R.A. Pederson2, Philip Weyman3, Peter K Weber1, Ulla Rasmussen2 and Christopher L Dupont4, (1)Lawrence Livermore National Laboratory, Livermore, CA, United States, (2)Stockholm University, Stockholm, Sweden, (3)J. Craig Venter Institute La Jolla, La Jolla, CA, United States, (4)J. Craig Venter Institute, La Jolla, CA, United States
 
Coupling new soil probes and high-resolution trace gas isotopomer measurement systems: evaluating feasibility in controlled soil columns (606515)
Juliana Gil-Loaiza1, Laura K Meredith1, Joseph R Roscioli2, Joanne H Shorter2 and Jordan E. Krechmer2, (1)University of Arizona, School of Natural Resources and the Environment, Tucson, AZ, United States, (2)Aerodyne Research Inc., Billerica, MA, United States
 
Quantifying the Effects of Warming and Ecosystem Change on Arctic Soil Microbial Communities (623289)
Jeffrey Ryan Propster, Northern Arizona University, Flagstaff, AZ, United States, Egbert Schwartz, Center for Ecosystem Science and Society, Flagstaff, AZ, United States, Michaela Hayer, Northern Arizona University, Center for Ecosystem Science and Society, Flagstaff, AZ, United States, Michelle C Mack, University of Florida, Gainesville, FL, United States and Bruce A Hungate, Northern Arizona University, Department of Biological Sciences and the Center for Ecosystem Science and Society, Flagstaff, AZ, United States
 
Production and fate of metal chelates in alkaline soils (624097)
John B Cliff1, Rene M. Boiteau2, Chuck Smallwood3, Ravi K Kukkadapu4, Mark Wirth5, Mark Engelhard6, Alice Dohnalkova1, Daniel E. Perea1 and Tamas Varga2, (1)Pacific Northwest National Laboratory, Environmental Molecular Sciences Laboratory, Richland, WA, United States, (2)Pacific Northwest National Laboratory, Richland, WA, United States, (3)Sandia National Laboratories, Albuquerque, United States, (4)Pacific Northwest National Lab, Richland, WA, United States, (5)Pacific Northwest National Laboratory, Richland, United States, (6)Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, United States
 
Applying Quantitative Stable Isotope Probing to Metagenomic Sequencing of Rhizosphere-Associated Microbes (627157)
Benjamin Koch1, Evan Starr2, Shengjing Shi3, Jillian F Banfield4, Steven Blazewicz5, Paul Dijkstra6, Mary Firestone7, Bruce A Hungate8, Erin E Nuccio5, Egbert Schwartz9, Bram Stone9 and Jennifer Pett-Ridge5, (1)Northern Arizona University, Center for Ecosystem Science and Society, Flagstaff, AZ, United States, (2)University of California Berkeley, Berkeley, CA, United States, (3)AgResearch, Palmerston North, New Zealand, (4)University of California, Berkeley, Berkeley, CA, United States, (5)Lawrence Livermore National Laboratory, Livermore, CA, United States, (6)Northern Arizona Univ, Flagstaff, AZ, United States, (7)Univ California Berkeley, Berkeley, CA, United States, (8)Northern Arizona University, Department of Biological Sciences and the Center for Ecosystem Science and Society, Flagstaff, AZ, United States, (9)Center for Ecosystem Science and Society, Flagstaff, AZ, United States
 
Linking Variation in Active Soil Microbial Communities with Edaphic Properties in Mediterranean Grasslands Along a Precipitation Gradient (627532)
Megan Foley1, Steven Blazewicz2, Dinesh Adhikari2, Karis J McFarlane2, Alex Greenlon3, Jennifer Pett-Ridge2 and Bruce A Hungate1, (1)Northern Arizona University, Department of Biological Sciences and the Center for Ecosystem Science and Society, Flagstaff, AZ, United States, (2)Lawrence Livermore National Laboratory, Livermore, CA, United States, (3)University of California Berkeley, Berkeley, CA, United States
 
Isotope tools scaling soil microbial ecology to biogeochemistry (629902)
Bruce A Hungate1, Paul Dijkstra2, Rebecca L Mau3, Michaela Hayer4, Egbert Schwartz3, Jennifer Pett-Ridge5 and Kirsten S Hofmockel6, (1)Northern Arizona University, Department of Biological Sciences and the Center for Ecosystem Science and Society, Flagstaff, AZ, United States, (2)Northern Arizona Univ, Flagstaff, AZ, United States, (3)Center for Ecosystem Science and Society, Flagstaff, AZ, United States, (4)Northern Arizona University, Center for Ecosystem Science and Society, Flagstaff, AZ, United States, (5)Lawrence Livermore National Laboratory, Livermore, CA, United States, (6)Pacific Northwest National Laboratory, Richland, WA, United States
 
Change Temporary Title for Abstract 632714 (632714)
Peter K Weber1, Alexa Nicolas2, Gareth George Trubl3, Erin E Nuccio1, Katerina Estera-Molina4, Mary Firestone5, Jennifer Pett-Ridge1 and Steven Blazewicz1, (1)Lawrence Livermore National Laboratory, Livermore, CA, United States, (2)University of California Berkeley, Berkeley, CA, United States, (3)University of Arizona, Tucson, AZ, United States, (4)University of California Berkeley, Berkeley, United States, (5)Univ California Berkeley, Berkeley, CA, United States
 
Mechanistic Elucidation of Uranium Isotope Fractionation in Biotic Reduction using Ab-initio Calculations (633814)
Ataru Sato, Minori Abe and Masahiko Hada, Tokyo Metropolitan University, Tokyo, Japan
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