P044-0002
Electronic Life-detection Instrument for Enceladus/Europa (ELIE)
Friday, 11 December 2020
Poster
Christopher E. Carr1, Daniel Duzdevich2, Jack W Szostak3, Sam Lee4, Masateru Taniguchi5, Takahito Ohshiro5, Yuki Komoto5, Gary Ruvkun6, Jason M Soderblom7 and Maria T Zuber8, (1)Georgia Institute of Technology Main Campus, Atlanta, GA, United States, (2)Howard Hughes Medical Institute, Boston, MA, United States, (3)Howard Hughes Medical Institute, Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA, United States, (4)Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, Cambridge, MA, United States, (5)Osaka University, Institute of Scientific and Industrial Research, Osaka, Japan, (6)Massachusetts General Hospital, Department of Molecular Biology, Boston, United States, (7)MIT, Cambridge, MA, United States, (8)Massachusetts Inst Tech, Cambridge, MA, United States
Abstract:
Habitable regions of Europa may include a subsurface ocean and transient liquid environments within its icy shell. Ocean-surface communication may occur on 1–2 million-year (My) timescales or even more rapidly in chaos regions. Any ice-entombed organisms could remain viable at near-surface depths (10–100 cm) over 1–10 ky. The proposed Europa Lander will target samples at depths >10 cm, potentially enabling recovery of viable organisms if sampling conditions are ideal. Any life there would likely represent a separate genesis event from Earth life. Life detection approaches should therefore not only target life
as we know it (contamination, common physicochemistry), but also
as we don’t know it, to lower the risk of false negatives.
We propose to target prebiotic, ancient, or extant life using a novel fully-electronic single-molecule detection strategy. Now in early development (PICASSO), the Electronic Life-detection Instrument for Enceladus/Europa (ELIE) instrument will utilize quantum electron tunneling between nanogap electrodes to interrogate the electronic structure of single molecules. Nanogaps are formed by breaking a gold nanowire embedded on a silicon chip. Bending is then used to control the gap size in the sub-nanometer regime. A molecule can be identified by its characteristic conductance and interaction time as a function of gap size.
This technology can detect and distinguish among amino acids, and detect RNA and DNA bases and short base sequences. The extrapolated limit of detection for single amino acids is ~200 ppt after 5 min of sampling (~1 pMol/g). Integrating upfront separation methods will enhance specificity and sensitivity. Our lab-bench prototype integrates a nanogap chip, low-noise amplifier, and a laptop for data processing. We target a ~1 kg flight instrument mass. ELIE will be able to measure two key biosignatures: the amino acid complexity distribution, and charged informational polymers, through to be universal for aqueous based life.
