Environmental DNA sequencing represents an increasingly critical tool for conducting rapid biodiversity assessments across diverse marine habitats. Recent high-throughput sequencing studies have focused on the detection of unseen species (from microbes to mammals), baseline biodiversity assessments in critical ecosystems (e.g. Kelp Forests and lease areas for deep-sea mining), and tracking anthropogenic impacts related to activities such as aquaculture and oil spills. However, many challenges still remain for incorporating new “–Omics” methodologies (metabarcoding, metagenomics) into routine biomonitoring and environmental management projects, including recovery of false positive/negatives, taxonomic bias in eDNA sequencing datasets, and the lack of standardized reference databases and bioinformatics pipelines. This session will highlight new scientific insights and technological advances from recent environmental –Omics studies focused on marine biodiversity and biomonitoring. Importantly, we will highlight interdisciplinary approaches spanning a broad range of taxonomic groups (microbial, invertebrate, and vertebrate studies), and emphasize links between “traditional” biomonitoring surveys and cutting-edge –Omics techniques.