We developed a novel approach for efficient functionalization of glass-type substrates with biocompatible layers. Our method allowed us to prepare self-assembled monolayers (SAMs), with protecting acetal groups, on surfaces of glass, quartz and silica. The novelty of our technique is the ability to deprotect the aldehyde groups under neutral conditions immediately prior to use. Deprotection of the SAMs is conducted under mild conditions which will not result in hydrolysis of Si-O-Si bonds. SAMs produced using this technique is treated with amine terminated poly ethylene glycol (PEG) under reductive amination conditions to form a highly packed PEG layers. These PEG-coated SAMs suppressed the nonspecific interaction between micrometer-size glass beads. We have successfully demonstrated immobilization of an enzyme, carbonic anhydrase, on the PEG surfaces, without compromising its activity. The activity of the enzyme immobilized on the PEG-coated surfaces was determined from spectrophotometric kinetic data. Storage of the substrates for extended periods of time did not compromise their catalytic properties resultant from the surface-bound enzymes.

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