| Single-Walled Carbon Nanotubes as near-Infrared Fluorescence and Raman Labels | ||
| Daniel A. Heller, Department of Chemistry, University of Illinois at Urbana-Champaign, 104 RAL, MC-712, 600 S. Mathews Ave., Urbana, IL 61801, Seunghyun Baik, School of Mechanical Engineering, Sungkyunkwan University, Suwon 440-746, South Korea, Thomas E. Eurell, Department of Veterinary Biosciences, University of Illinois at Urbana-Champaign, 3223 VMBSB MC-002, 2001 S Lincoln, Urbana, IL 61802 and Michael S. Strano, Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 104 RAL, MC-712, 600 S. Mathews Ave., Urbana, IL 61801 Nucleic acid-encapsulated single-walled carbon nanotubes show Raman scattering and near-infrared fluorescence emission within live mammalian cells in long-term culture. Raman scattering of nanotubes was observed within live murine 3T3 and myoblast stem cells, remaining visible for 3 months and also after the H&E staining procedure. Nanotube fluorescence is in the near-infrared region and does not photobleach under prolonged excitation, permitting continuous monitoring of the cells for experiments lasting hours. Fluorescence emission remained visible after a week in culture and exhibited spectral changes within the cells, leading to possible optical sensors. Nanotubes were taken into endosomes and remained after multiple cell divisions, allowing long-term sensing and labeling experiments. Extended Abstract Status: Not Uploaded | ||