Preliminary Program subject to change
15C03 Advances in Protein Expression and Post-Translational Modification
OverviewIncreasing demand for recombinant proteins has focused research on techniques for improving protein expression and controlling post-translational processing. We invite papers on all aspects of protein expression including novel cell systems, expression vectors, and culture conditions. Submissions dealing with post-translational processing including glycosylation, editing and secretion are also invited. Papers may deal with any cell type including bacteria, yeast, mammalian, insect and plant cell cultures.
Primary SponsorBioengineering (15c)

Chair

Matthew P. DeLisa
Assistant Professor
Cornell University
School of Chemical and Biomolecular Engineering
254 Olin Hall
Ithaca, NY 14853
Phone Number: 607-254-8560
Fax Number: 607-255-9166
Email: md255@cornell.edu

CoChair

Xuejun (Sherry) Gu
Bioprocess R&D
Eli Lilly & Company


Email: GU_XUEJUN@Lilly.com
Breaking the Degeneracy of the Genetic Code
Inchan Kwon and David A. Tirrell, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125
Ligand-Induced Protein Splicing: a General Way of Achieving Post-Translational Regulation of Protein Activity in Vivo
David W. Wood, Princeton University, A213 EQUAD, Princeton, NJ 08544 and Georgios Skretas, Princeton University, Olden st., Princeton, NJ 08544
Strategies for Expanding the Repertoire of Proteins That Can Be Displayed on the Outer Surface of E. Coli
Jae-Young Kim1, Gina Cremona1 and Matthew P. DeLisa2, (1)School of Chemical and Biomolecular Engineering, Cornell University, 120 Olin Hall, Ithaca, NY 14853, (2)Cornell University, School of Chemical and Biomolecular Engineering, 254 Olin Hall, Ithaca, NY 14853
Regulation of Cd47-Sirpa Interactions by Post-Translational Modifications
Shyamsundar Subramanian, Eric T. Boder and Dennis E. Discher, Chemical & Biomolecular Engineering, University of Pennsylvania, 220 South 33rd Street, Room 112 Towne Bldg., Philadelphia, PA 19104-6391
Intermission
Programmable, Ligand-Controlled Riboregulators of Gene Expression
Travis S. Bayer, Department of Biochemistry and Molecular Biophysics, California Institute of Technology, 1200 E. California Blvd., MC 210-41, Pasadena, CA 91125 and Christina Smolke, Division of Chemistry & Chemical Engineering, CalTech, Pasadena, CA 91125
Effect of Culture Conditions on the Glycosylation of Human Secreted Alkaline Phosphatase (Seap) Expressed in Tobacco Nt1 Cell Suspension Cultures
Alejandro Becerra-Arteaga, School of Chemical and Biomolecular Engineering, Cornell University, 120 Olin Hall, Ithaca, NY 14853 and Michael L. Shuler, Cornell University, 120 Olin Hall, Ithaca, NY 14853-5201
Improved Membrane Protein Expression Using Cell-Free Protein Synthesis
Jessica J. Wuu and James R. Swartz, Chemical Engineering, Stanford University, MC 5025, Stanford, CA 94305

Food, Pharmaceutical & Bioengineering Division