Preliminary Program subject to change
TA001 Advances in Proteomic Analysis: Electrophoresis-based Approaches (Part 1)
OverviewSystematic studies of gene expression patterns play a vital role in understanding the complex interactions associated with the global response of cells, tissues, and organisms to stimuli or mutations. While recent developments have allowed these patterns to be investigated at an unprecedented level of detail, further advances are needed in order to fully illuminate the interplay among the many factors governing cellular response. Specifically, new technologies are needed with the capability of providing quantitative information with greatly enhanced levels of sensitivity and throughput. This session will focus on the development of novel techniques to address these limitations, including ultra-sensitive protein detection, high-throughput antibody creation and optimization, the development of protein-detecting microarrays, and other related technologies.
Primary SponsorTopical A - American Electrophoresis Society Annual Meeting (TA)

Chair

Feng Wang
Principal Scientist
Procter & Gamble Pharmaceuticals
8700 Mason-Montgomery Road
Mason, OH 45040
Phone Number: 513-6223967
Fax Number: 513-6220978
Email: wang.f@pg.com

CoChair

Ray Grant
Principal Scientist
Procter & Gamble Company
11810 East Miami River Road
Ross, OH 45061
Phone Number: 513-627-2179
Email: grant.ra@pg.com
Plenary: Prefractionation of Serum Proteins Using Microscale Solution Isoelectrofocusing to Enhance Detection of Low Abundance Proteins for Biomarker Discovery
Hsin-Yao Tang, Lynn A. Echan, Nadeem Ali-Khan, WonA Joo, Glenn Tan and David W. Speicher, The Wistar Institute, 3601 Spruce St, Room 152, Philadelphia, PA 19104
a High-Throughput Information Added Proteomic Separation Strategy Using Free Flow Electrophoresis and Tandem Mass Spectrometry
Hongwei Xie1, Sricharan Bandhakavi1, Nelson Rhodus2, Robert J. Griffin3, John V. Carlis4 and Timothy J. Griffin1, (1)Biochemistry, Molecular Biology & Biophysics, University of Minnesota, 321 Church ST. SE, 6-155 Jackson Hall, Minneapolis, MN 55455, (2)Dental School, University of Minnesota, Minneapolis, MN 55455, (3)Department of Therapeutic Radiology, University of Minnesota, Minneapolis, MN 55455, (4)Department of Computer Science, University of Minnesota, Minneapolis, MN 55455
Two Dimensional Electrophoresis in Liquid Media: Advances Using the Protein Profiler™ for the Separation and Quantification of Fluorescence-Labeled Proteins and Peptides
John E. Wiktorowicz and Alex Kurosky, Human Biological Chemistry & Genetics, The University of Texas Medical Branch, 301 Universtiy Blvd., Galveston,, TX 77555-0635
Enhanced Molecule Detection in Capillary Electrophoresis Using Label Free Intrinsic Imaging
John F. Hassard, deltaDOT Ltd, 13 Princes Gardens, South Kensington, London, United Kingdom
High Speed and High Sensitivity Two Dimensional Capillary Electrophoresis with Laser Induced Fluorescence of Barrett's Esophageal Cells
Jim Kraly, Megan Jones and Norman J. Dovichi, Chemistry, University of Washington, Bagley Hall, University of Washington, Seattle, WA 98195
Multistage Electrophoresis Applications in Protein Analysis
Paul W. Todd1, Lara M. Deuser1, Nathan A. Thomas1, D. James Morré2, Dorothy M. Morré3 and Nicole McClain2, (1)Space Hardware Optimization Technology, Inc, 7200 Highway 150, Greenville, IN 47124-9515, (2)Medicinal Chemistry and Molecular Pharmacology, Purdue University, 201 S. University Street, West Lafayette, IN 47907, (3)Department of Foods and Nutrition, Purdue University, Stone Hall, Rm. G-1E, 700 W. State St., West Lafayette, IN 47907

Topical A - American Electrophoresis Society Annual Meeting