Correlation between the Protein Binding Strength on Immobilized Metal Affinity Chromatography and the Surface Accessibility of Its Histidine
Lakshmi P. Pathange, Virginia Tech, 200 Seitz Hall, Blacksburg, VA 24061, David R. Bevan, Virginia Tech, 201 Fralin Biotech Center, Blacksburg, VA 24060, Timothy J. Larson, Virginia Tech, 202 Fralin Biotech Center, Blacksburg, VA 24060 and Mike (Chenming) Zhang, Dept. of Biological Systems Engineering, Virginia Tech, 210 Seitz Hall, Blacksburg, VA 24061 |
Utilization of Gibbs Free Energy to Optimize the pH and Imidazole Operating Conditions of Immobilized Metal Affinity Chromatography for Protein C Purification
James J. Lee1, Duane F. Bruley2 and Kyung A. Kang1, (1)Chemical Engineering, University of Louisville, Eastern Parkway, Louisville, KY 40292, (2)Chemical and Biochemical Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250 |
Salt Concentration and Loading Effects on Protein Unfolding during Hydrophobic Interaction Chromatography
Jace L. Fogle1, Yunzhi Xiao1, Abigail H. Laurent2, Jie Chen3, Steven M. Cramer4, Todd M. Przybycien5, John P. O'Connell1 and Erik Fernandez6, (1)Department of Chemical Engineering, University of Virginia, 102 Engineers' Way, Charlottesville, VA 22904, (2)Department of Chemical Engineering, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, PA 15213-3890, (3)Department of Chemical and Biological Engineering, Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Ricketts Bldg., Troy, NY 12180, (4)Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Ricketts Bldg., Troy, NY 12180, (5)Carnegie Mellon University, 5000 Forbes Ave., Pittsburgh, PA 15213-3890, (6)University of Virginia, 102 Engineers' Way, Charlottesville, VA 22904 |
Understanding Lactalbumin Structural Change in Hydrophobic Interaction and Reverse Phase Chromatography
Abigail H. Laurent1, Jie Chen2, Jace L. Fogle3, Yunzhi Xiao3, Steven M. Cramer4, John P. O'Connell5, Erik Fernandez5 and Todd M. Przybycien6, (1)Department of Chemical Engineering, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, PA 15213-3890, (2)Department of Chemical and Biological Engineering, Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Ricketts Bldg., Troy, NY 12180, (3)Department of Chemical Engineering, University of Virginia, 102 Engineers' Way, Charlottesville, VA 22904, (4)Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Ricketts Bldg., Troy, NY 12180, (5)University of Virginia, 102 Engineers' Way, Charlottesville, VA 22904, (6)Carnegie Mellon University, 5000 Forbes Ave., Pittsburgh, PA 15213-3890 |