Preliminary Program subject to change
TA002 Advances in Proteomic Analysis: Electrophoresis-based Approaches (Part 2)
OverviewElectrophoresis technology continues to be a vital tool for quantitative proteomic analysis. 2-D gel electrophoresis techniques allow multiple gene expression patterns to be simultaneously monitored in order to identify and characterize complex cellular interactions. This session will focus on the development of these proteomic technologies and their application to biotechnology. Of particular interest are papers describing advances in gel-free protein separations, novel protein stains, methods of analyzing membrane proteins, and mass spectroscopic methods for resolving mixtures of peptides. Papers are also sought that present research on the application of proteomics to the study of bacterial, animal, and plant cell cultures, and especially proteomic analysis of post-translational modifications.
Primary SponsorTopical A - American Electrophoresis Society Annual Meeting (TA)

Chair

Feng Wang
Principal Scientist
Procter & Gamble Pharmaceuticals
8700 Mason-Montgomery Road
Mason, OH 45040
Phone Number: 513-6223967
Fax Number: 513-6220978
Email: wang.f@pg.com

CoChair

Ray Grant
Principal Scientist
Procter & Gamble Company
11810 East Miami River Road
Ross, OH 45061
Phone Number: 513-627-2179
Email: grant.ra@pg.com
Plenary: an Effective Skeletal Muscle Pre-Fractionation Method to Remove Abundant Structural Proteins for Optimized Two Dimensional Gel Electrophoresis
Bradley B. Jarrold, Jeffrey P. DeMuth, Kenneth D. Greis, Thomas M. Burt and Feng Wang, Discovery Core Technologies, Procter & Gamble Pharmaceuticals, Inc., Health Care Research Center, 8700 Mason-Montgomery Road, Mason, OH 45040-9317
Use of Sequential Staining to Characterize Post-Translational Modifications
Ivan C. Gerling1, Jian Wu2, Michael J. Pabst3 and Nataliya I. Lenchik2, (1)Deparment of Medicine, and The Center of Genomics and Bioinformatics, University of Tennessee Health Science Center, VAMC Research 151, 1030 Jefferson Avenue, Memphis, TN 38104, (2)Medicine, University of Tennessee Health Science Center, VAMC Research 151, 1030 Jefferson Avenue, Memphis, TN 38104, (3)Peridontology, University of Tennessee Health Science Center, VAMC Research 151, 1030 Jefferson Avenue, Memphis, TN 38104
Sample Compatibility Screening and Optimization of Separation Protocols Using Mini Two-Dimensional Gels
Detlef Schumann and Michael Wyder, Department of Genome Science, University of Cincinnati, Genome Research Institute, 2180 E. Galbraith Road, Cincinnati, OH 45237
Use of Two-Dimensional Electrophoresis for Studying Protein Phosphorylation
Nancy Kendrick, Jon J Johansen and Matthew Hoelter, Kendrick Labs Inc, 1202 Ann St, Madison, WI 53713
Interspecies Cyanobacterial Proteome Comparison and Characterization Using Two-Dimensional Gel Electrophoresis and Gel-Free Shotgun Proteomics
Martin E. Barrios-Llerena, University of Sheffield, Chemical and Process Engineering, Mappin street, Sheffield, S1 3JD, UK, Kenneth F. Reardon, Colorado State University, Department of Chemical Engineering, Fort Collins, CO 80523-1370 and Phillip C. Wright, The University of Sheffield, Biological & Environmental Systems Group, Department of Chemical and Process Engineering, Mappin Street, Sheffield, S1 3JD, UK
Quantitative Proteomic Analysis of E. Coli Using Isobaric Tags
Kunal Aggarwal1, Leila H. Choe1, Zsofia Franck1 and Kelvin H. Lee2, (1)School of Chemical and Biomolecular Engineering, Cornell University, 120 Olin Hall, Ithaca, NY 14853, (2)School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853-5201

Topical A - American Electrophoresis Society Annual Meeting