Preliminary Program subject to change
02F01 Particle and Cell Separations in Bioprocessing
OverviewThis session examines the performance and development of physical separations to remove parental biomass from liquid solutions containing biochemical products in crude or intermediate form. Biomass suspensions can derive from fermentation of cells of plants or mammals or from solvent extraction of transgenic plants. Bioprocessing aims to remove solid-phase cells, growth media, bacteria and other contaminating particulates that would interfere with subsequent purification of the product. Some form of filtration or sedimentation dominates the means and efficient separation can require two stages where the solids span several orders of magnitude in size or their concentration is high. Primary solids are removed by centrifugal sedimentation or cake filtration driven by pressure, vacuum or centrifugal force. The resulting liquid is clarified or polished by pressure-driven membrane microfiltration, cartridge filtration, depth filtration including the use of filter aids, or centrifugal sedimentation at high g-force. Biomass is frequently washed with solvent to further recover valuable solutes by displacement or repeated dissolution (reslurry). Topics of interest include all these various technologies applied in bioprocessing.
Primary SponsorFluid-Particle Separations (02f)

Chair

Ray M Collins


Email: rmcollins@dow.com
A Rational Approach to Design an Efficient Primary Recovery Process for a Recombinant Protein from Microbial Biomass
Anant Patkar1, Subrata Sen1 and Wu Chen2, (1)Biotechnology, Core R&D, The Dow Chemical Company, 5501 Oberlin Dr., San Diego, CA 92121, (2)Engineering Sciences, Core R&D, The Dow Chemical Company, B-1402, 2301 N. Brazonsport Blvd., Freeport, TX 77541
Separation of (Sub-)Micron Bioparticles by Selective Flotation
P. Van Hee1, RGJM Van der Lans1 and LAM Van der Wielen2, (1)Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, Delft, NL-2628 BC, Netherlands, (2)Department of Biotechnology, Delft University of Technology, Julianalaan 67, Delft, NL-2628 BC, Netherlands
Broth Conditions Determining Specific Cake Resistance during Microfiltration of Bacillus Subtilis
Kevin M. Graves1, Meng Heng2, Glenn Rozeboom2 and Charles E. Glatz3, (1)Chemical and Biological Engineering, Iowa State University, 2114 Sweeney Hall, Ames, IA 50011, (2)Genencor International Inc, 925 Page Mill Road, Palo Alto, CA 94304, (3)Department of Chemical and Biological Engineering, Iowa State University, 2114 Sweeney Hall, Ames, IA 50011
Optimum Technology for Separating Biomaterials from Extracts
Raymond M. Collins, Engineering and Process Sciences, Dow Chemical Co., 845 Building, Midland, MI 48667

Separations Division