Preliminary Program subject to change
15B15 Advances in Protein Structure, Function, and Stability I
OverviewThis session seeks submissions in the areas of experimental, theoretical, and computational approaches to rational prediction, design, and/or optimization of protein structure, stability, and function in laboratory and processing settings relevant to biopharmaceutical products in general. Studies may include structural analysis and engineering of proteins, creation or optimization of function, thermodynamics and dynamics of protein conformational stability, issues in folding or misfolding, degradation via physical and chemical routes, or related concerns in intracellular expression and refolding, purification, and final product formulation. Preference will be given to papers dealing with strategies that are relevant to commercial and biological systems and also provide insights into the molecular pathways or mechanisms controlling behavior.
Primary SponsorPharmaceuticals (15b)

Chair

Jeffrey J. Gray
Asst. Professor
Johns Hopkins
Chemical and Biomolecular Engineering
3400 N. Charles St.
Baltimore, MD 21218
Phone Number: (410) 516-5313
Fax Number: (410) 516-5510
Email: jgray@jhu.edu

CoChair

Christopher J. Roberts
Assistant Professor
Department of Chemical Engineering
University of Delaware
Newark, DE 19716
Phone Number: 302-831-0838
Fax Number: 302-831-1048
Email: roberts@che.udel.edu
Protein Switches Created by Non-Homologous Recombination
Gurkan Guntas1, Jing Liang2, Jin Ryoun Kim3, Thomas J. Mansell3, Jason Boock3 and Marc Ostermeier3, (1)Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 North Charles St., Baltimore, MD 21218, (2)Program in Molecular and Computational Biophysics, Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218, (3)Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218
An Engineered Chimeric Enzyme for Use in Drug Sensing, Discovery and Development
David W. Wood, Princeton University, A213 EQUAD, Princeton, NJ 08544 and Georgios Skretas, Princeton University, Olden st., Princeton, NJ 08544
De Novo Engineering of a Bacterial Disulfide Isomerase
Laura Segatori, Silvia Arredondo and George Georgiou, Chemical Engineering, University of Texas at Austin, 1 University Station C0400, Austin, TX 78712
Directed Evolution of Homing Endonuclease with Altered DNA Sequence Specificity
Zhilei Chen, Biophysics and Computational Biology, University of Illinois, 221 Roger Adams Lab, 600 S. Mathews Ave., Urbana, IL 61801 and Huimin Zhao, University of Illinois, 215 Roger Adams Lab, Box C3, 600 S. Mathews Ave., Urbana, IL 61801
Intermission
Engineering of High Affinity Binding Peptides Using N-Terminal Bacterial Display
Jeffrey J. Rice, Aaron Schohn and Patrick S. Daugherty, Chemical Engineering, UCSB, Engineering II, Room 3357, Santa Barbara, CA 93106-5080
Computational Prediction of the Mab 806-Egfr Complex Structure by Combining Protein Docking with Computational and Experimental Mutagenesis
Arvind Sivasubramanian, Chemical & Biomolecular Engineering, Johns Hopkins, Chemical and Biomolecular Engineering, 3400 N. Charles St., Baltimore, MD 21218, Ginger Chao, Massachusetts Institute of Technology, 77 Massachusetts Ave. 56-478, Cambridge, MA 02139, K. Dane Wittrup, MIT, 77 Mass. Ave. 66-552, Cambridge, MA 02139 and Jeffrey J. Gray, Johns Hopkins, Chemical and Biomolecular Engineering, 3400 N. Charles St., Baltimore, MD 21218
Measurements of Protein Folding and Unfolding Kinetic Pathways Using a Microfluidic Approach
Jinkee Lee1, Joshua Ziperstein2 and Anubhav Tripathi1, (1)Brown University, Division of Engineering, Box D, Providence, RI 02912, (2)Biomedical Engineering, Brown University, Division of Engineering, Box D, Providence, RI 02912

Food, Pharmaceutical & Bioengineering Division