Pooling of Enzyme Libraries for High Throughput Biocatalyst Development
Karen M. Polizzi1, Cody U. Spencer2, Ichiro Matsumura3, Anshul Dubey1, Monal Patel3, Jay H. Lee4, Matthew J. Realff4 and Andreas S. Bommarius5, (1)School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Dr, Atlanta, GA 30332, (2)School of Computer Science, Georgia Institute of Technology, 311 Ferst Dr, Atlanta, GA 30332, (3)Biochemistry, School of Medicine, Emory University, Atlanta, GA 30322, (4)Chemical and Biomolecular Engineering, Georgia Institute of Technology, Ford Environmental Science & Technology Building 311 Ferst Drive, N.W., Atlanta, GA 30332, (5)Georgia Institute of Technology, School of Chemical Engineering, 315 Ferst Drive N.W., Parker H. Petit Biotechnology Institute, Room 3310, Atlanta, GA 30332-0363 |
Optimizing Mixing in Micro-Bioreactors Used for High Throughput Screening of Suitable Biocatalysts in Industrial Fermentation
Xiaonan Li1, Marcel Ottens1, Gijs W.K. van Dedem1, Luuk AM Van der Wielen1, Michiel van Leeuwen1, Walter van Gulik1, Josef J. Heijnen1, E.E. Krommenhoek2, J.G.E. Gardeniers2 and A. van den Berg2, (1)Department of Biotechnology, Delft University of Technology, Julianalaan 67, Delft, 2628 BC, Netherlands, (2)MESA+, Enschede, Netherlands |
Development of Rigorous Distance Bounds for Improved Protein Structure Prediction
Scott R. McAllister and Christodoulos A. Floudas, Chemical Engineering, Princeton University, Dept of Chemical Engineering; A215 Engineering Quadrangle, Princeton, NJ 08544 |
Incorporation and Labeling of an Aliphatic Ketone in Recombinant Proteins
James A. Van Deventer, Chemical Engineering, California Institute of Technology, MC 210-41, 1200 E. California Blvd., Pasadena, CA 91125, A. James Link, Caltech, MC 210-41 1200 E. California Blvd., Pasadena, CA 91106, Yi Tang, Chemical Engineering, University of California, Los Angeles, Los Angeles, CA 90096, Pin Wang, Chemical Engineering and Material Science, University of Southern California, 925 Bloom Walk, HED-216, Los Angeles, CA 90089-1211 and David A. Tirrell, Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125 |
A Novel Systems Engineering Approach for in Silico Sequence Selection in De Novo Protein Design
Ho Ki Fung, Department of Chemical Engineering, Princeton University, Engineering Quadrangle, Olden Street, Princeton, NJ 08544 and Christodoulos A. Floudas, Chemical Engineering, Princeton University, Engineering Quadrangle, Princeton, NJ 08540 |
High-Throughput Biocatalysis on Microarrays for Synthesis and Screening of Small Molecule Therapeutics
Disha Ahuja1, Lakshmi Santhanam2, Sarah Brooks1, Sylvain Antoniotti3 and Jonathan S. Dordick1, (1)Department of Chemical & Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, (2)Division of Cardiology Department of Medicine, The Johns Hopkins Medical Institutions, Baltimore, MD 21209, (3)Laboratoire Synthèse Organique Sélective et Produits Naturels, ENSCP, Paris, France |
Stabilization of Interface-Binding Chloroperoxidase
Ravindrabharathi Narayanan and Ping Wang, Chemical Engineering, University of Akron, 301 Whitby hall, Akron, OH 44325-3906 |
Integration of Relaxing Substrate Inhibition and Competitive Inhibition of Lipoxygenase by Dmf in Aerobic Catalysis
Yun Fang, Yafen Su, Yongmei Xia, Yan Cai, Qilei Zhang, Hongli Xu and Hui Long, School of Chemical and Material Engineering, Southern Yangtze University, Wuxi, China |
Solvent Strategies of Asymmetric Reducing Synthesis Ethyl (R) -2-Hydroxy-4-Phenylbutyrate Catalyzed by Yeast
Yugang Shi, Yun Fang, Yongmei Xia, Hongping Wu and Feng Li, School of Chemical and Material Engineering, Southern Yangtze University, Wuxi, China |
Blue Fluorescent Protein Variants with Enhanced Brightness from a Computationally Designed Library
Marco A. Mena, Paul H. Bessette, Karen Y. Dane and Patrick S. Daugherty, Department of Chemical Engineering, UC Santa Barbara, Engineering II Rm 3357, Santa Barbara, CA 93106-5080 |
Residue-Rotamer-Reduction for Fast Protein Side-Chain Conformation Prediction
Wei Xie, Chemical and Biomolecular Engineering, University of Illinois, 600 South Mathews Avenue, Urbana, IL 61801 and Nick Sahinidis, University of Illinois, Department of Chemical and Biomolecular Engineering, 600 South Mathews Avenue, Urbana, IL 61801 |
Toward the Transition State: Further Docking Studies on Family 47 Alpha-1,2-Mannosidases
Chandrika Mulakala and Peter J. Reilly, Chemical and Biological Engineering, Iowa State University, 2114 Sweeney Hall, Ames, IA 50011-2230 |
Combined Simulation Approach of Atomistic and Continuum Models for the Thermodynamics of Lysozyme Crystals
Jaeeon Chang, Chemical engineering, University of Delaware, 150 Academy St, Newark, DE 19716, Abraham Lenhoff, Department of Chemical Engineering, University of Delaware, 226 Colburn Lab, Newark, DE 19716-3110 and Stanley Sandler, University of Delaware, Department of Chemical Engineering, Newark, DE 19716 |
Quantification of Binary Diffusion in Protein Crystals
Luuk AM Van der Wielen1, Rajamani Krishna2, Adrie J. J. Straathof3, Aleksandar Cvetkovic4 and Cristian Picioreanu3, (1)Department of Biotechnology, Delft University of Technology, Julianalaan 67, Delft, 2628 BC, Netherlands, (2)Department of Chemical Engineering, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV, Amsterdam, 1018 WV, The Netherlands, (3)Dept of Biotechnology, TU Delft, Julianalaan 67, Delft, 2628 BC, The Netherlands, (4)Dept of Biotechnology, TU Delft, Julianalaan 67, Delft, 2628 BC, Netherlands |
Molecular Simulation and Energy Landscape Analysis of Mechanical Unfolding of the Titin Protein
Dan Lacks1, Nhat-Hang Duong2 and Nathan Duff2, (1)Case Western Reserve University, Dept. of Chemical Engineering, Cleveland, OH 44106, (2)Chemical Engineering, Case Western Reserve University, Dept. of Chemical Engineering, Cleveland, OH 44106 |
Directed Evolution of Homing Endonuclease with Novel DNA Sequence Specificity
Zhilei Chen, Biophysics and Computational Biology, University of Illinois, 221 Roger Adams Lab, 600 S. Mathews Ave., Urbana, IL 61801 and Huimin Zhao, University of Illinois, 215 Roger Adams Lab, Box C3, 600 S. Mathews Ave., Urbana, IL 61801 |
Cell-Free Production of Proteins Requiring Disulfide Bonds
Kurtis Knapp and James R. Swartz, Stanford University, Department of Chemical Engineering, Stanford, CA 94305-5025 |
Peptide Microarray-Based Mapping of Prion Transmission Barriers
Peter M. Tessier and Susan Lindquist, Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142 |