Tuesday, 1 November 2005
291ae
Minimizing the Surface Effect on Pcr in Pdms-Glass Chips by Dynamic Passivation
Yongmei Xia
1, Zhishan Hua
2, Erdogan Gulari
2, Onnop Srivannavit
2, and
Ayse Bilge Ozel2. (1) School of Chemical and Material Engineering, Southern Yangtze University, Wuxi, China, (2) Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109
Dynamic passivation of surfaces of PDMS-glass PCR chips using polyethylene glycol (PEG) or polyvinylpyrrolidone (PVP) was achieved in a conventional thermocycler. The effects of molecular weight of the polymers and the polymer concentration in PCR mixture on surface passivation were investigated. Efficiency of the dynamic passivation follows the order: PVP10000 > PVP55000, PEG8000> PEG10000> PEG400. 0.4% and 0.025% (w/v) are the suitable final polymer concentrations in PCR mixture. Competitive adsorption could be the principal activity while the effect of polymers on Taq polymerase is a minor function during the PCR.
See more of #291 - Poster Session: Nanoscale Science and Engineering (TF015)
See more of Topical F – 5th Topical Conference on Nanoscale Science and Engineering
See more of The 2005 Annual Meeting (Cincinnati, OH)