Tuesday, 1 November 2005
291o
Single-Walled Carbon Nanotube Detection of Ion-Mediated DNA Polymorphism
Daniel A. Heller1, Esther S. Jeng
2, Tsun-Kwan Yeung
3, Brittany M. Martinez
2, Anthonie E. Moll
2, Joseph B. Gastala
4, and Michael S. Strano
5. (1) Department of Chemistry, University of Illinois at Urbana-Champaign, 104 RAL, MC-712, 600 S. Mathews Ave., Urbana, IL 61801, (2) Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 104 RAL, MC-712, 600 S. Mathews Ave., Urbana, IL 61801, (3) Department of Physics, University of Illinois at Urbana-Champaign, 104 RAL, MC-712, 600 S. Mathews Ave., Urbana, IL 61801, (4) Department of Chemical and Biomolecular Engineering, University of Illinois, Urbana-Champaign, 600 S. Mathews, Urbana, IL 61821, (5) Chemical and Biomolecular Engineering, University of Illinois, Urbana-Champaign, 600 S. Mathews, Urbana, IL 61821
The transition of DNA secondary structure from the B to Z conformation was detected via modulation of the intrinsic near-infrared fluorescence of single-walled carbon nanotubes. A reversible shift in emission energy of DNA-encapsulated nanotubes was found to closely match the thermodynamics of the transition of unbound DNA. A 30-mer oligonucleotide on the nanotube surface behaves as if it were shortened by 1/6 due to the propagation length of the B-Z transition. The system motivates new near-infrared biosensors capable of operation in strongly scattering or absorbing media, and we demonstrate the optical detection of the B-Z transition from localized complexes within live mammalian cells.
See more of #291 - Poster Session: Nanoscale Science and Engineering (TF015)
See more of Topical F – 5th Topical Conference on Nanoscale Science and Engineering
See more of The 2005 Annual Meeting (Cincinnati, OH)