A Sub- and Supercritical Fluid Chromatography System is developed for separation of chiral isomers of flurbiprofen, a non-Steroidal Anti-Inflammatory Drug (NSAID). S-isomer is used for various pain treatments such as Rheumatoid Arthritis and Osteoarthritis while R-isomer is used in cancer research. The column utilized for this method was a Pirkle brush-type Whelk-O 1 analytical column packed with 10µm particles. Carbon dioxide is used as a mobile phase with isopropanol and sec-butanol employed as a modifier. The temperature was varied to provide sub and supercritical conditions for the alcohol – carbon dioxide mixture. The effect of temperature on the separation factor, peak resolution and retention time is studied. The adsorption isotherms are used to abstract isotherm information for scale up and synthesis of operating policies for fractionation through simulated moving bed. Isotherms were obtained at the optimum conditions for separation. Elution techniques such as Elution by a Chromatographic Point and peak maxima as well as frontal analysis technique were used in order to determine isotherms for single component adsorption as well as isotherms for competitive adsorption at finite concentrations of the solutes.