| Systematic studies of gene expression patterns play a vital role in understanding the complex interactions associated with the global response of cells, tissues, and organisms to stimuli or mutations. While recent developments have allowed these patterns to be investigated at an unprecedented level of detail, further advances are needed in order to fully illuminate the interplay among the many factors governing cellular response. Specifically, new technologies are needed with the capability of providing quantitative information with greatly enhanced levels of sensitivity and throughput. This session will focus on the development of these proteomic technologies and their application to biotechnology. Of particular interest are papers describing advances in gel-free protein separations, novel protein stains, methods of analyzing membrane proteins, mass spectroscopic methods for resolving mixtures of peptides, high-throughput antibody creation and optimization, the development of protein-detecting microarrays, and other related technologies. Papers are also sought that present research on the application of proteomics to the study of bacterial, animal, and plant cell cultures, and especially proteomic analysis of post-translational modifications. |
Characterisation of Hep-G2 Conditioned Medium for Enhanced Mesoderm Differentiation of Es Cells: Application to Skeletal Tissue Engineering
Yunyi Kang1, Yu-shik Hwang2 and Athanasios Mantalaris1, (1)Department of Chemical Engineering and Chemical Technology, Imperial College London, South Kensington Campus, London, SW7 2AZ, United Kingdom, (2)Tissue Engineering & Regenerative Medicine Centre, Chelsea & Westminster Campus, Imperial College London, 369 Fulham Road, London, United Kingdom |
De Novo Peptide Identification Via Mixed-Integer Linear Optimization and Tandem Mass Spectrometry
Christodoulos A. Floudas and Peter A. DiMaggio, Chemical Engineering, Princeton University, Princeton, NJ 08544 |
Dynamic Quantitative Analysis of the Nucleolar Proteome Using an Isobaric Mass Tagging Approach
Bing Xie1, Eric R. Kershnar2, Craig Parman1, Rixin Wang2, Ed Papacoda2, Jennifer Maloney2, Cesar E. Guerra1, Paul M. Kaplan2, Brian T. Chait3, Paul M. Lizardi4 and Martin J. Mattessich2, (1)Biochemistry, PerkinElmer LAS, 549 Albany Street, Building 100-1, Boston, MA 02118, (2)Proteomics, Agilix Corporation, 11 Research Drive, Suite 3, Woodbridge, CT 06525, (3)The Rockefeller University, 1230 York Avenue, New York, NY 10021, (4)Department of Pathology, Yale University School of Medicine, 310 Cedar Street LH 214, New Haven, CT 06520 |
Enhancing Shotgun Proteomics Data Analysis with Bioinformatics Tools: Application to Cyanobacterial Proteomics
Martin E. Barrios-Llerena1, Saw Yen Ow1, Poh Kuan Chong2, Chee Sian Gan3, Ambrosius P. Snijders1, Kenneth F. Reardon4 and Phillip C. Wright1, (1)Department of Chemical and Process Engineering, The University of Sheffield, Biological & Environmental Systems Group, Sheffield, S1 3JD, United Kingdom, (2)Chemical and Process Engineering, University of Sheffield, Mappin Street, Sheffield, South Yorkshire S1 3JD, UK, (3)University of Sheffield, Mappin Street, Sheffield, South Yorkshire S1 3JD, UK, (4)Department of Chemical and Biological Engineering, Colorado State University, 1370 Campus Delivery, Fort Collins, CO 80523 |
Novel Selective Fluorescent Gel Stains for Improved Detection of Phosphoproteins
Susan E. Kramer, Eva Golenko, Alvydas Mikulskis and Wayne F. Patton, R&D, PerkinElmer, Boston, MA 02118 |
Optimization of Chip-Based Infusion Nanoelectrospray Tandem Mass Spectrometry for the Rapid Analysis of Complex Proteomes
Ana G. Pereira-Medrano1, Alistair Sterling2, Ambrosius P. Snijders1, Kenneth F. Reardon3 and Phillip C. Wright1, (1)Department of Chemical and Process Engineering, The University of Sheffield, Biological & Environmental Systems Group, Sheffield, S1 3JD, United Kingdom, (2)Advion Biosciences Ltd, Rowan House, 28 Queens Road, Norwich, NR9 3DB, United Kingdom, (3)Department of Chemical and Biological Engineering, Colorado State University, 1370 Campus Delivery, Fort Collins, CO 80523 |
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