Preliminary Program subject to change
T3002 Advances in Proteomics: New Technologies II
OverviewSystematic studies of gene expression patterns play a vital role in understanding the complex interactions associated with the global response of cells, tissues, and organisms to stimuli or mutations. While recent developments have allowed these patterns to be investigated at an unprecedented level of detail, further advances are needed in order to fully illuminate the interplay among the many factors governing cellular response. Specifically, new technologies are needed with the capability of providing quantitative information with greatly enhanced levels of sensitivity and throughput. This session will focus on the development of these proteomic technologies and their application to biotechnology. Of particular interest are papers describing advances in gel-free protein separations, novel protein stains, methods of analyzing membrane proteins, mass spectroscopic methods for resolving mixtures of peptides, high-throughput antibody creation and optimization, the development of protein-detecting microarrays, and other related technologies. Papers are also sought that present research on the application of proteomics to the study of bacterial, animal, and plant cell cultures, and especially proteomic analysis of post-translational modifications.
Primary SponsorTopical 3: 2006 Annual Meeting of the American Electrophoreses Society (AES) (T3)

Chair

Feng Wang
Discovery Core Technologies
Procter and Gamble Pharmaceuticals, Inc.
Health Care Research Center
8700 Mason-Montgomery Road
Mason, OH 45040-9317
Phone Number: 513 622-3967
Fax Number: 513-622-0978
Email: wang.f@pg.com
Phosphopeptide Analysis Using a Planar Electrochromatography/ Thin-Layer Chromatography Separations Platform Coupled to Orthogonal Maldi-Tof Mass Spectrometry
Wayne F. Patton1, Venkateswarlu Panchagnulu2, Alvydas Mikulskis2 and Ira S. Krull3, (1)PerkinElmer LAS, Boston, MA 02118, (2)Biochemistry, PerkinElmer LAS, Boston, MA 02118, (3)Chemistry, Northeastern University, 360 Huntington Avenue - 102 Hurtig Hall, Boston, MA 02115
Pressurized Planar Electrochromatography: a New Tool for Proteomics?
David Nurok1, Allyson L. Novotny1, Robert E. Santini2, Randall W. Replogle3 and Gregory L. Hawkins2, (1)Department of Chemistry and Chemical Biology, Indiana University - Purdue University Indianapolis, 402 North Blackford Street, Indianapolis, IN 46202, (2)Chemistry, Purdue University, 560 Oval Drive, West Lafayette, 47907, (3)Purdue University, 560 Oval Drive, West Lafayette, 47907
Proteomic Analysis of Immunogenic Proteins in Xenogeneic Heart Valve Bioscaffolds
Leigh G. Griffiths1, E. Christopher Orton1 and Kenneth F. Reardon2, (1)Veterinary Teaching Hospital, Colorado State University, Fort Collins, CO 80523, (2)Department of Chemical and Biological Engineering, Colorado State University, 1370 Campus Delivery, Fort Collins, CO 80523
A Closer Look at E. Coli-Derived 6-Deoxyerythronolide B Biosynthesis
Brett A. Boghigian, Yong Wang and Blaine A. Pfeifer, Chemical and Biological Engineering, Tufts University, 4 Colby Street, Medford, MA 02155
Proteome Analysis of Pseudomonas Putida during Biodegradation of High Concentration of Benzoate:Activation of the Meta Pathway and Physiological Responses
Kai Chee Loh and Bin Cao, Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117576, Singapore
2d Gel Phosphoprotein Western Blotting
Nancy Kendrick, Jon Johansen and Matthew Hoelter, Kendrick Labs Inc, 1202 Ann St, Madison, WI 53713

Topical 3: 2006 Annual Meeting of the American Electrophoresis Society (AES)