Preliminary Program subject to change
T3009 Advances in CE and Microdevice Technology for Genomic Analysis
OverviewMicrofluidic-based DNA and protein separation systems are beginning to emerge from the research laboratory and appear as commercially available products for use in a variety of genomic analysis applications. If sufficient miniaturization can be achieved, these microfabricated systems will enjoy a tremendous cost advantage over today’s conventional macroscale systems, thereby ensuring a central role in future genomic analysis efforts such as the ambitious goal of sequencing a genome for $1,000 or less. Strategies to develop improved sieving media based on a variety of polymeric and non-polymeric materials incorporating uniform and reproducible microstructures promise to generate tremendous improvements in the achievable level of separation performance. In addition, separation matrices composed of nanofabricated structures constructed directly on the surfaces of silicon, glass, and plastic substrates offer exciting possibilities in terms of exerting precise control over pore size and sieving properties. Novel techniques to analyze DNA and proteins by directly probing the motion of single molecules either through nanoscale fluidic channels or through membrane channel nanopores also show enormous potential. Chemical engineers continue to make important contributions in these areas, and we invite abstracts related to any aspect of the development or study of biomolecule separation technology at the microscale, or on miniaturized devices.
Primary SponsorTopical 3: 2006 Annual Meeting of the American Electrophoreses Society (AES) (T3)

Chair

Malgorzata A. Witek
Department of Chemistry
Louisiana State University
Baton Rouge, LA 70803
Phone Number: (225) 578-3563
Fax Number: (225) 578-3458
Email: mwitek@lsu.edu

CoChair

Adrienne R. Minerick
Assistant Professor
Dave C. Swalm School of Chemical Engineering
Mississippi State University
Box 9595
Mississippi State, MS 39762
Phone Number: 662-325-7323
Fax Number: 662-325-2482
Email: minerick@che.msstate.edu
How Is It Possible to Sequence 600 Bases of DNA in 6.5 Minutes? The Central Role of Carefully Engineered Polymer Networks and Coatings in Microchip Electrophoresis
Christopher P. Fredlake and Annelise E. Barron, Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208
Nanostructural Characterization of Photopolymerized Polyacrylamide Gels for DNA Electrophoresis
Jian Wang, Chemical Engineering, Texas A&M University, 3122 TAMU, College Station, TX 77843 and Victor M. Ugaz, Department of Chemical Engineering, Texas A&M University, 3122 TAMU, College Station, TX 77843
DNA Sequencing and Separation in Free Solution Using Engineered Drag-Tags
Robert J. Meagher, Chemical & Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, Russell D. Haynes, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, Jennifer Lin, Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road - TECH E136, Evanston, IL 60208, Jennifer A. Coyne, Chemical and Biological Engineering, Northwestern Uni, 2145 Sheridan Road, Evanston, IL 60208, Jong-In Won, Chemical Engineering, Hongik University, Seoul, South Korea and Annelise E. Barron, Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208
Microchip-Based Electrophoretic Analysis of Low Abundant Unknown Mutations Using Site-Specific Nicking and Ligation Enzymes
Rondedrick Sinville1, Hanna Pincas2, Francis Barany2 and Steven A. Soper1, (1)Chemistry, Louisiana State University, Center for BioModular Microsystems and Department of Chemistry, Choppin Hall Rm. 232, Baton Rouge, LA 70803, (2)Molecular Biology, Weill Medical College of Cornell University, New York, NY 10021
Sacrificial Layer Methods for Making High-Performance Capillary Electrophoresis Microchips
Adam T. Woolley, Ryan T. Kelly, Bridget A. Peeni and Yi Li, Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602
Optimal Separation Times with an Orthogonal Electrical Field in a Cylindrical Capillary
Pablo Vergara, Chemical Engineering and Material Sciences, University Catolica del Norte, Avenida Angamos 0610, Antofagasta, Chile, Mario A. Oyanader, Universidad Catolica del Norte, Avenida Angamos, Antofagasta, 0610, Chile and Pedro E. Arce, Department of Chemical Engineering, Tennessee Tech University, Prescott Hall Room 214, Cookeville, TN 38505

Topical 3: 2006 Annual Meeting of the American Electrophoresis Society (AES)