- 5:00 PM
267f

Adsorption and Desorption of Lysozyme on Thermosensitive Nano-Sized Magnetic Particles and Its Conformational Changes

Nabila Shamim, Kus Hidajat, Liang Hong, and M. Shahab Uddin. Department of Chemical and Biomolecular Engineering, National University of Singapore, 10 Kent Ridge Crescent, Singapore, 119260, Singapore

">   and desorption of lysozyme on thermosensitive nano magnetic particles and its conformational changes were studied in this work. Poly (N-isopropylacrylamide) (PNIPAM) coated nano magnetic particles were used as adsorbents. Adsorption of lysozyme on these PNIPAM coated particles was carried out at different temperatures and pH values. It was observed that maximum amount of lysozyme was adsorbed at pH 11 which is the isoelectric point of lysozyme; and at a temperature (40oC) higher than the lower critical solution temperature of the polymer (32oC). The adsorption is believed to be due to the hydrophobic effect of the polymer. Desorption of lysozyme from nano magnetic particles was achieved using NaH2PO4 (pH 4.0) and NaSCN (pH 6.0) as desorbents. However, higher desorption efficiencies was achieved when prior adsorption was done at lower temperature. The conformational change of the lysozyme desorbed by NaH2PO4 was small, while the lysozyme desorbed by NaSCN underwent significant conformational change as measured by the intrinsic fluorescence. 81.42 % activity was retained in the desorbed enzyme for desorption by NaH2PO4. Keywords: Nano-magnetic particles, N-isopropylacrylamide; lysozyme; hydrophobic; conformational change; enzyme activity.

b



A

G

p

6

7

'

g

"""""³³ h!3 h^D

h!3 H* hd H*hd

h{g

h{Sq H*h{Sq

h^D H*h?

hD

hkd5

hfB

h ?

h!3

h^D

hgv 5h ? h ? 5hgv D 









=!"#$%

  P a r a g r a p h

F o n t face="Symbol">³ R  N o r m a l

 

4  l 4 

a



( k (

 L i s t

>*B*ph

[) _# Z*' .) (P) /4, Z- E. ' / ^1/ 3 [3 {4 kd5 C8 ? r&B ,B &)C ^D "dD CF ,J K L t M YN oO Q HMQ WOR aAS

Z vZ e@Z ZD] [K^ E3` a -b WAb ~c ",i ~lj bm {Sq Cr 4s 4u Bv %} ~ 7 4 P[ W 1J "7 U' o' io w 29 :  G ^Y x 9£ ^ 'Xµ xµ : ) n F v u2 .³ \³ x [m 6 5  5m ~ #  " .

<  ' pc m 5' ]' ? I~ !3 _ d x N Q{  " s Dv o {g 0  M* { "i X] D R @

 N e w

R o m a n

T i m e s

N e w

R o m a n

5 

 a n d

D e s o r p t i o n

o f

l y s o z y m e

o n

t h e r m o s e n s i t i v e

n a n o - s i z e d

m a g n e t i c

p a r t i c l e s

a n d

i t s

c o n f o r m a t i o n a l

c h a n g e s I Oh +'³0















$



8



D



P



d

p



|





x























x

Adsorption and Desorption of lysozyme on thermosensitive nano-sized magnetic particles and its conformational changes



 Office Word

@font-family:Symbol[

@font-family:Symbol^Ll@font-family:Symbol^Ll





z



B

>









NUS 











 and Desorption of lysozyme on thermosensitive nano-sized magnetic particles and its conformational changes 







Title 



D

 



8







_AdHocReviewCycleID 



_EmailSubject 

_AuthorEmail 



_AuthorEmailDisplayName 



_ReviewingToolsShownOnce 





GX'

Abstract for APCChE conference





g0203689@nus.edu.sg 



Nabila Shamim





!

"

#

%

&

'

(

)

*

+

.

R o o t

E n t r y Office Word Document

MSWordDoc 

Word.Document.8 9qface="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"face="Symbol"


See more of #267 - Biocomposites (08F04)
See more of Materials Engineering and Sciences Division

See more of The 2006 Annual Meeting