| Length Dependent Uptake Of Single-Wall Carbon Nanotubes By Human Lung Cells | ||
| Jeffrey A. Fagan1, Matthew L. Becker2, Barry J. Bauer2 and Erik K. Hobbie2, (1)Polymers Division, National Institute of Standards and Technology, 100 Bureau Dr., Gaithersburg, MD 20899-8542, (2)National Institute of Standards and Technology, 100 Bureau Dr., Gaithersburg, MD 20899-8542 Live human lung fibroblasts (IMR-90) were exposed to controlled quantities of length-sorted single wall carbon nanotubes (SWNTs), and the uptake of the SWNTs is shown to be length dependent. Cell mortality is shown to result from the uptake of shorter nanotubes and is correlated with both SWNT length and concentration. The intrinsic band gap fluorescence of individual semi-conducting SWNTs stabilized via either single-stranded DNA or deoxycholate surfactant was exploited to optically observe the interactions between the lung cells and length-fractionated SWNTs using near-infrared (NIR) fluorescence microscopy in a microfluidic flow platform. The NIR optical measurements are used to identify potential uptake mechanisms and quantify the kinetics of the interaction. Extended Abstract Status: Not Uploaded | ||