Preliminary Program subject to change
15C03 Advances in Protein Expression and Post-Translational Modification
OverviewIncreasing demand for recombinant proteins has focused research on techniques for improving protein expression and controlling post-translational processing. We invite papers on all aspects of protein expression including novel cell systems, expression vectors, and culture conditions. Submissions dealing with post-translational processing including glycosylation, editing and secretion are also invited. Papers may deal with any cell type including bacteria, yeast, mammalian, insect and plant cell cultures.
Primary SponsorBioengineering (15c)

Chair

Eric T. Boder
Assistant Professor
Chemical and Biomolecular Engineering
University of Pennsylvania
231 S. 34th Street, 176
Philadelphia, PA 19104
Phone Number: (215) 898-5658
Fax Number: 215-573-6416
Email: boder@seas.upenn.edu

CoChair

Matthew DeLisa
Chemical Engineering
Cornell University
304 Olin Hall
Ithaca, NY 14853
Phone Number: 607-254-8560
Fax Number: 607-255-9166
Email: md255@cornell.edu
Improved Expression of Recombinant Protein Using a Cysteine Protease and Chitinase Deficient Bombyx Mori Multiple Nucleopolyhedrovirus Bacmid System in Silkworm Larvae
Masato Hiyoshi1, Takahiro Abe1, Tatsuya Kato1 and Enoch Y. Park2, (1)Department of Applied Biological Chemistry, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka, 422-8529, Japan, (2)Graduate School of Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka, 422-8529, Japan
Effects Of Microcarrier Culture And Temperature Shift On Productivity And Glycosylation Of Recombinant Proteins Produced In Chinese Hamster Ovary Cells
Jong Hyun Nam1, Fuming Zhang1, Myriam Ermonval2, Robert J. Linhardt3 and Susan Sharfstein1, (1)Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Biotech 2nd Floor, Troy, NY 12180, (2)Département de biologie cellulaire et Infection, Institut André Lwoff, Villejuif & Institut Pasteur, Paris, France, (3)Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, 110 8th Street, Biotech 2nd Floor, Troy, NY 12180
Heterologous Expression Of Fungal Polyketide Synthases
Suzanne M. Ma, Chemical and BioMolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, Los Angeles, CA 90095 and Yi Tang, Chemical and BioMolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, 5331 Boelter Hall, Los Angeles, CA 90096
A Versatile Selection Technology For Intracellular Protein-Protein Interactions Mediated By A Unique Bacterial Hitchhiker Transport Mechanism
Dujduan Waraho, Chemical and Biomolecular Engineering, Cornell University, 120 Olin Hall, Cornell University, Ithaca, NY 14850 and Matthew DeLisa, Chemical Engineering, Cornell University, 304 Olin Hall, Ithaca, NY 14853
Break
High-Throughput Cell-Based Screening for Therapeutic Antibody Process Development and An Investigation of the Biochemical Network for Specific Mab Productivity
Tiffany D. Rau, Chemical Engineering Department, Vanderbilt University, VU Station B #351604, 2301 Vanderbilt Place, Nashville, TN 37235-1604 and R. Robert Balcarcel, Cell and Tissue Technologies, BD, 2350 Qume Drive, San Jose, CA 95131-1807
Transcriptional And Metabolic Responses In High-Cell Density Cultures During Temperature-Induced Synthesis Of Recombinant Protein In Escherichia Coli: A Scale-Down Study
Luis Caspeta1, Noemí Flores2, Francisco Bolívar2 and Octavio T. Ramírez1, (1)Medicina Molecular y Bioprocesos, Instituto de Biotecnologia, Av. Universidad #2001, Col. Chamilpa C.P. 62210, Cuernavaca, Morelos, Mexico, (2)Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia, Av. Universidad #2001, Col. Chamilpa C.P. 62210, Cuernavaca, Morelos, Mexico
Effect Of Glycosylation On The Stability Of Transgenic Antibodies
Andrea Cascio, CBE, UMR, 1870 miner circle, UMR, rolla, MO 65409 and Daniel Forciniti, cbe, UMR, 1870 miner circle, UMR, rolla, MO 65409

Food, Pharmaceutical & Bioengineering Division