| Systematic studies of gene expression patterns play a vital role in understanding the complex interactions associated with the global response of cells, tissues, and organisms to stimuli or mutations. While recent developments have allowed these patterns to be investigated at an unprecedented level of detail, further advances are needed in order to fully illuminate the interplay among the many factors governing cellular response. Specifically, new technologies are needed with the capability of providing quantitative information with greatly enhanced levels of sensitivity and throughput. This session will focus on the development of these proteomic technologies and their application to biotechnology. Of particular interest are papers describing advances in gel-free protein separations, novel protein stains, methods of analyzing membrane proteins, mass spectroscopic methods for resolving mixtures of peptides, high-throughput antibody creation and optimization, the development of protein –detecting microarrays, and other related technologies. Papers are also sought that present research on the application of proteomics to the study of bacterial, animal, and plant cell cultures, an especially proteomic analysis of post-translational modifications. |
Quick, Easy, Reproducible Protein Isoelectric Fractionation Using Protein Forest digital ProteomechipTM Technology
Aaron Sin, James Dasch, William Skea and Russell Garlick, Protein Forest, Inc., 100 Beaver St, Suite 210, Belmont, MA 02453 |
An Orthogonal Affinity Capture and Detection Strategy for Identifying and Characterizing Phosphoproteins Directly from Sds-Polyacrylamide Gels: Inorganic Chemistry to the Rescue!
Alvydas Mikulskis, Linan Song, Alla Bogdanova, Hao Xie, Eva Golenko, Yang Wang and Wayne F. Patton, Biochemistry, PerkinElmer Life & Analytical Sciences, 940 Winter Street, Waltham, MA 02451 |
Instrumentation and Protocols for Optimized Separation of Peptides by Pressurized Planar Electrochromatography, with Subsequent Direct Interrogation of Analytes by Tandem Mass Spectrometry
Wayne F. Patton1, David Bourdon2, Peter Jackson3, Mei Wang1, Yang Wang1, Linan Song1, Bob Krug2 and Steven Liebold2, (1)Biochemistry, PerkinElmer Life & Analytical Sciences, 940 Winter Street, Waltham, MA 02451, (2)R&D, PerkinElmer Life &Analytical Sciences, 2200 Warrenville Road, Downers Grove, IL 60515, (3)R&D, PerkinElmer Life & Analytical Sciences, Chalfont Road, Seer Green, Beaconsfield, Bucks, United Kingdom |
A Hybrid Methodology For Peptide Identification Via Mixed-Integer Linear Optimization, Local Alignment Database Search And Tandem Mass Spectrometry
Peter A. DiMaggio, Chemical Engineering, Princeton University, Princeton, NJ 08544 and Christodoulos A. Floudas, Department of Chemical Engineering, Princeton University, Engineering Quadrangle, Princeton, NJ 08544 |
Integrated Top-Down And Bottom-Up Strategy For Characterizing Intact Proteins And Their Modifications
Ljiljana Pasa-Tolic, Biological Sciences Division, Pacific Northwest National Laboratory, 902 Battelle Boulevard, P.O. Box 999 M.S. K8-98, Richland, WA 99352 |
15N Metabolic Labeling And Tandem Mass Spectrometry: Tools For The Proteome Characterization Of The Facultative Psychrophile pedobacter Cryoconitis Via Ortholog Searching And de Novo Sequencing
Ana G. Pereira-Medrano, Department of Chemical and Process Engineering, The University of Sheffield, Biological & Environmental Systems Group, Sheffield, S1 3JD, United Kingdom, Rosa Margesin, Institut für Mikrobiologie, Leopold-Franzens-Universität, Technikerstraße 25, Innsbruck, A-6020, Austria and Phillip C. Wright, The University of Sheffield, Biological & Environmental Systems Group, Sheffield, S1 3JD, United Kingdom |
Identification Of Phosphotyrosine-Containing Proteins By Immunoprecipitation And Mass Spectrometry
Nancy Kendrick, Jon J Johansen and Matthew Hoelter, Kendrick Labs Inc, 1202 Ann St, Madison, WI 53713 |
Fabrication Of Cell-Free Expressed Protein Libraries For Proteomics: The Use Of T-RNA Engineering, Photocleavable Linkers And Multiplexed Protein Production On Beads
Mark J. Lim, AmberGen Inc., 100 Beaver Street, Room 269, Waltham, MA 02453, Sadanand Gite, Molecular Diagnostics, AmberGen Inc., 100 Beaver Street, Waltham, MA 02453, Ziying Liu, Proteomics, AmberGen Inc., 100 Beaver Street, Waltham, MA 02453 and Kenneth J. Rothschild, Department of Physics and Photonics Center - Molecular Biophysics Laboratory, Boston University, Boston, MA 02215 |
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