| This session will focus on issues related to the culture, differentiation, and proliferation of cells for use in cellular therapies. Cells and tissues of interest include embryonic stem cells, mesenchymal stem cells and adult progenitor cells such as skin, nerve, or liver progenitors, as well as primary cells such as pancreatic islets, hepatocytes, blood, skin, and nerve cells. Important issues include: (1) Regulation of stem/progenitor cell expansion and directed differentiation, (2) Development of culture media and control of the cellular microenvironment (PO2, pH, shear, and ECM), (3) Development of culture systems that mimic key aspects of the stem/progenitor cell niche, (4) Quality control and validation assays for cell characterization, (5) Development of bioreactors and microfluidics for automated differentiation or proliferation of cells, and (6) Improved recovery and purification of desired cell populations. |
Stem Cell Self-Renewal In A Suspension Bioreactor Without Serum
Daniel E. Kehoe, Lye T. Lock and Manolis S. Tzanakakis, Chemical and Biological Engineering, State University of New York at Buffalo, Buffalo, NY 14260 |
Activin Alters The Kinetics Of Endoderm Induction In Embryonic Stem Cells Cultured On Collagen Gels
Natesh Parashurama1, Yaakov Nahmias2, Cheul Cho2, Daan Van Poll2, Arno Tilles2, Francois Berthiaume2 and Martin L. Yarmush1, (1)Department of Chemical Engineering, Rutgers, The State University of New Jersey, Massachusetts General Hospital, Harvard Medical School, 51 Blossom St, Boston, MA 02114, (2)Massachusetts General Hospital, Harvard Medical School, 51 Blossom street, Boston, MA 02114 |
Tuning Hydrogel Modulus And Ligand Density Independently To Control Adult Neural Stem Cell Self-Renewal And Differentiation
Krishanu Saha, Univ. of California-Berkeley, 201 Gilman Hall, Berkeley, CA 94720, Kevin E. Healy, Univ. of California-Berkeley, Depts. of Bioengineering and Materials Science, 370 Hearst Memorial Mining Building, Berkeley, CA 94720 and David V. Schaffer, University of California at Berkeley, Department of Chemical Engineering, 201 Gilman Hall, Berkeley, CA 94720-1462 |
Interstitial Flow Modulates 3-D Human Mesenchymal Stem Cell Construct Development
Feng Zhao, Katelyn Sellgren and Teng Ma, Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, 2525 Pottsdamer Street, Tallahassee, FL 32310 |
Increased Erk Activation and Intracellular Nadh Levels May Explain Nicotinamide-Mediated Increases in Megakaryocyte Size, Ploidy and Proplatelet Formation
Lisa M. Giammona1, Eleftherios T. Papoutsakis2 and William M. Miller1, (1)Chemical and Biological Engineering, Northwestern University, 2145 N. Sheridan Rd., Tech E136, Evanston, IL 60208, (2)Dept. of Chemical Engineering, Delaware Biotechnology Institute, 15 Innovation Way, Newark, DE 19711 |
Co-Culture Of 3T3-L1 Cells With Human Umbilical Vein Endothelial Cells
Ning Lai, Chemical and Biological Engineering, Tufts University, 4 Colby Street, Science and Technology Center, Medford, MA 02155 and Kyongbum Lee, Chemical and Biological Engineerig, Tufts University, 4 Colby Street, Science and Technology Center, Medford, MA 02155 |
Optimization Of The Generation Of Alloreactive Depleted T-Cell Transplants: The Challenge Of Nonspecific Binding
Ying Xiong1, Xiaodong Tong1, Sherif Farag2, Maciej Zborowski3 and Jeffrey J. Chalmers4, (1)Chemical and Biomolecular Engineering, The Ohio State Unversity, 140 W 19th Ave, Columbus, OH 43210, (2)Bone Marrow and Stem Cell Transplantation, Indiana University School of Medicine, 635 Barnhill Drive, Room 224G, Indianapolis, IN 46202, (3)Department of Biomedical Engineering, Cleveland Clinic Foundation, 9500 Euclid Ave, Cleveland, OH, (4)Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Avenue, Columbus, OH 43210 |
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