| Separation unit operations make up a critical portion of biologics downstream processes. Complexity inherently associated with biologics manufacture such as product heterogeneity and host impurities presents process challenges not typically encountered in small-molecule separations. With bioreactor titers increasing steadily over the last several years, production cost and operational pressures are shifting further to downstream. In addition, biological metabolites are still produced in relatively low amounts. The development of cost- and time-effective bioseparation and concentration unit ops is thus key to efficient downstream processes. This session will focus on highlighting the latest advances in bioseparations and downstream processing, aimed at cost reduction, improved efficiency, and shortend development time. Topics to be covered include, but are not limited to: Centrifugation; Crystallization, evaporation, and precipitation; Membrane-based separations; Adsorption and ion exchange; Extraction; Expanded-bed chromatography. |
Disc Stack Centrifugation 2L To 20,000L Scale-Up Evaluation For Mammalian Cell Clarification: Optimization Of Bioreactor Harvest Conditions For Combined Cell Removal And Mab Purification Capabilities
Jonathan Karl Romero1, James Chrostowski1, Philippe de Vilmorin1 and John Smelko2, (1)Process Development Engineering, BiogenIdec Inc, 15 Cambridge Center, Cambridge, MA 02142, (2)Manufacturing Sciences, BiogenIdec Inc, Raleigh, NC 27709 |
An Ultra-Scale down Study of the Separation of Flocculated E. Coli Extract in a Disc-Stack Centrifuge
Alex Berrill1, Sa Ho2 and Daniel Bracewell1, (1)Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, United Kingdom, (2)Pfizer Global R&D, 700 Chesterfield Village Parkway West, Chesterfield, MO 63017 |
Simple and Efficient Downstream Bioprocessing of Virus-Like Particles Using Cell Free Reactors
Yap Pang Chuan1, D. I. Lipin2, L. Lua1 and A. P. J. Middelberg1, (1)Centre for Biomolecular Engineering, Australian Institue of Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Australia, (2)Centre for Biomolecular Engineering, Department of Chemical Engineering, The University of Queensland, Brisbane, Australia |
Dowex 1x4-400 Treatment Of A Recombinant e. Coli Extract: Removal Of Colored Impurities, Catechols, And Endotoxin
John T. Herberg, Alan K. Hunter and Sa V. Ho, Global Biologics, Pfizer, 700 Chesterfield Parkway West, Chesterfield, MO 63017 |
Adsorption Of A Small Non-Enveloped Virus Using A Trimeric Peptide
Caryn L. Heldt, Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, Patrick V. Gurgel, ProMetic Life Sciences, 8168 Montview Rd, Mont-Royal, QC H4P 2L7, Canada, Lee-Ann Jaykus, Department of Food Science, North Carolina State University, Raleigh, NC 27695 and Ruben Carbonell, Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27606 |
Affinity Chromatography Of Cell Culture Derived Vaccinia Virus
Michael W. Wolff1, Corina Venzke2, Anke Zimmermann3, Sara Post Hansen4, René Djurup4, Rene Faber5 and Udo Reichl3, (1)Bioprocess Engineering, Max Planck Institute, Sandtorstrasse 1, Magdeburg, 39106, Germany, (2)Otto-von-Guericke-Universität, Universitätsplatz 2, Magdeburg, 39106, Germany, (3)Bioprocess Eng., Max Planck Institute, Sandtorstrasse 1, Magdeburg, 39106, Germany, (4)Bavarian Nordic A/S, Bøgeskovvej 9, Kvistgård, 3490, Denmark, (5)Biotechnology Division, Sartorius Biotech GmbH, Weender Landstrasse 94-108, Goettingen, 37075, Germany |
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