Supercritical CO2 extraction was used to fractionate and enrich high value phospholipids from sewage sludge. Lecithin was selected as a model to evaluate supercritical CO2 extraction lipids and phospholipids from swage sludge. Neat supercritical CO2 was used to extract oil from soybean lecithin and then supercritical CO2 with 5 – 15 wt% methanol as a co-solvent was used to extract phospholipids from de-oil soybean lecithin were performed. Gas chromatography (GC) fitted with a flame ionization detector (FID) was used to quantitatively analysis the fatty acid composition of extract. Fourier transform infrared spectroscopy (FTIR)-attenuated total reflectance (ATR) and nuclear magnetic resonance (NMR) was used to qualitatively and semi-quantitatively analysis lipids and phospholipids in the extract. Neat CO2 was used to extract the oil from sewage sludge. The sludge was then extracted with supercritical CO2 over a range of temperatures, with 5 – 15 wt% methanol as a cosolvent. Phosphorus analysis was also performed to quantitatively analysis phosphorus content in the sample and extracts. The effect of extraction temperature, level of co-solvent employed and extraction pressure were examined.