A New High-Throughput Screening Method for Determining Active and Enantioselective Hydrolases

A new high-throughput screening method using fluorescein sodium salt as indicator to obtain hydrolases with high enantioselectivity is developed, which is demonstrated to be sensitive and reliable. Because of the application of small amount of optically pure enantiomers, screening large libraries of enzymes is allowed at low cost and in short time.