Abstract: Electrokinetically Driven DNA Dynamics in Nanochannel/Microwell Array (2009 Annual Meeting)

Monday, November 9, 2009: 4:45 PM

Canal A (Gaylord Opryland Hotel)

Orin L. Hemminger, Chemical and Biomolecular Engineering, Ohio State University, Columbus, OH

Pouyan E. Boukany, Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus,, OH

Xin Hu, NSEC center for Affordable Nanoengineering of Polymer Biomedical Devices (CANPBD), The Ohio State University, Columbus, OH

Nan-rong Chiou, Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH

Ly James Lee, The Ohio State University, Columbus, OH

Recently, we developed a simple and low-cost method to produce polymeric nanochannel/microwell arrays, which can be easily connected to microscale inlets/outlets to form a nanofluidic device. Such device has potential for gene delivery and DNA separation. The nanochannel/microwell array was fabricated in ethylene glycol dimethylacrylate (EGDMA). Hydrophobicity/Hydrophilicity and zeta potential of the channel wall can be adjusted by acid treatment. The periodic length of nanochannels ranged from 1 to 10 um with diameter of 10-200 nm. The microwells with different size and shape can be used to pre-stretch large DNA molecules and to adjust the electric field strength in nanochannels. Using spin-disk dynamic confocal microscopy, we investigate the transport behavior of various sizes of DNAs under different periodic nanoscale confinements by direct flow visualization, upon applications of pre-specified electric field strengths. The experimental observations are compared with coarse-grain Brownian dynamic simulation.

Extended Abstract: File Not Uploaded

See more of this Session: DNA Analysis in Microfluidic & Nanofluidic Devices
See more of this Group/Topical: Topical 3: 2009 Annual Meeting of the American Electrophoresis Society (AES)