PC12 Cell-Based Integrated Device with Dual Nanoporous Membranes for Parkinson Treatment

To investigate the feasibility of dual nanoporous membranes cell-based device entrapping with PC12 cells for Parkinson's disease treatment, in vitro study about this integrated device for maintenance cells viability and dopamine controlling release were carried out first. This integrated device consists of two capsules and each of them has nanoporous membrane for immunoprotection when application in vivo. PC12 cells were cultured in microcapsule and proliferated forming 3-D cell cluster. The cell viability was measured by live/dead staining and dopamine concentration was canalized using HPLC. The results showed this cell-based device has both controlled release therapeutic product and immunoprotection function.