This preliminary program is subject to change.

Advances in Protein Expression and Post-Translational Modification


Description:
This session will highlight advances in protein expression in a wide variety of hosts including bacteria, yeast, insect cells, mammalian cells, and plant cells. Advances in cell-free protein expression are also appropriate for this session. The session will also focus on novel methods for the trafficking and display of proteins within cells as well as research on the engineering of expression hosts for improved protein expression. In addition to the focus on protein expression, the session will also include papers on the development of in vivo and in vitro techniques for improved posttranslational modification of proteins, including glycosylation, editing and secretion.

Sponsor:
Bioengineering


Chair:
A. James Link
Princeton University
Chemical Engineering
A208 Engineering Quadrangle
Princeton, NJ 08540
Phone Number: 6092587191
Email: ajlink@princeton.edu

Co-Chair:
Lydia Contreras

Papers:
S-Adenosyl Methionine, Cysteine, Tyrosine, Iron, and Sulfide Stimulate in Vitro [FeFe] Hydrogenase Activation in a Heterologous E. Coli System
Jon M. Kuchenreuther, Stanford University; James A. Stapleton, Stanford University; James R. Swartz, Stanford University
Secretory Pathway Analysis of High Productivity in Mammalian Cell Culture through Genomics and Proteomics
Patrick M. Hossler, Abbott Bioresearch Center; Ivan Correia, Abbott Bioresearch Center; Sean McDermott, Abbott Bioresearch Center; Paul Jung, Abbott Laboratories; Paul Kroeger, Abbott Laboratories; Christof Schulz, Abbott Bioresearch Center; David Ouellette, Abbott Bioresearch Center; Lincoln Muhoro, Abbott Bioresearch Center; Boris Labkovsky, Abbott Bioresearch Center; Czeslaw Radziejewski, Abbott Bioresearch Center; Li Malmberg, Abbott Bioresearch Center; John Fann, Abbott Bioresearch Center
Proetomics-Guided Assessment of the Cellular Responses of Prokaryotic CELLS Over-Producing Recombinant Glycosylated Proteins
Phillip C. Wright, The University of Sheffield; Jagroop Pandhal, The University of Sheffield; Josselin Noirel, The University of Sheffield
Disulfide Stabilization of Noncovalent Quaternary Structures On the Yeast Surface
Kok-Hong Lim, State University of New York at Buffalo; Sri Madabhushi, State University of New York at Buffalo; Jasdeep Mann, State University of New York at Buffalo; Sriram Neelamegham, State University of New York at Buffalo; Sheldon Park, State University of New York at Buffalo
Understanding Translocation Bottlenecks for Mammalian G-Protein Coupled Receptors Heterologously Expressed in Saccharomyces Cerevisiae
Michelle A. O'Malley, Massachusetts Institute of Technology; Özge Can, University of Delaware; Anne Skaja Robinson, University of Delaware
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division