Cellular Viability After Electrostatic Deposition of Electrosprayed Nanoparticles

Nanotechnology-based gene and drug delivery strategies suffer from lack of methods to synthesize, characterize, and deliver highly uniform and precisely sized nano-vectors. Here we report the efforts on implementation of a novel method of electrostatic deposition of electrosprayed nanoparticles on cultured cells in vitro. The method employs a nanoparticle aqueous solution, which is first electrosprayed to produce highly charged mists of charged droplets that then can be electrostatically driven towards cellular surface. Multiple factors including intensity of electric field, deposition time, nanoparticles composition,size and concentration could affect cell survival.This study was focused on finding optimal experimental conditions for successful delivery of nanoparticles without compromising viability and morphology of cultured cells.