Development of a Microdevice-Based Human Mesenchymal Stem Cell-Mediated Drug-Delivery System

Development of a Microdevice-based Human Mesenchymal Stem Cell-mediated Drug-Delivery System

Junfei Xia^a, Ang-Chen Tsai^a, Teng Ma^a, Jingjiao Guan^a,b

^a Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University

^b Integrative NanoScience Institute, Florida State University

 

Abstract

Cell-mediated drug delivery allows to harness unique abilities of living cells to achieve controlled delivery of drugs, but traditional cell-mediated drug delivery techniques suffer drawbacks such as risk of intracellular degradation of drug payload or low drug loading capacity. These limitations can be potentially overcome by attaching a disk-shaped drug-laden microparticle termed microdevice to the external surface of a cell. On the other hand, human mesenchymal stem cell (hMSC) is a primary candidate for next-generation cell therapies. It is therefore an ideal cell type for cell-mediated drug delivery. In this presentation, we introduce production and characterization of a novel microdevice-based hMSC-mediated drug-delivery system for the first time. In particular, we present in vitro studies on how microdevice attachment affects viability, migration, stemness, capability of forming spheroid of the hMSCs. Successful development of this microdevice-based hMSC-mediated drug delivery system may lead to a new therapies for treating many human diseases.

Keywords: cell-mediated drug delivery, human mesenchymal stem cell, drug delivery