The Interdigitated Electrodes Stimulation of the Proliferation and Differentiation of Living Cells

The electric and magnetic fields were reported to be useful on improving cell growth. Here we report a simple method of fabrication of self-doped sulfonated polyaniline (SPAN)-based interdigitating electrodes (SPAN-IDEs) which is applied on cell stimulation. The electrodes are fabricated through a layer-by-layer deposition of SPAN copolymers and in situ polymerization on PET film, which were used for murine bone marrow stromal cells (BMSCs) and pre-osteoblasts cells (MC3T3-E1). The samples were evaluated by alkaline phosphatase (ALP) activity and Von Kossa staining, which gave us promising results for SPAN-IDEs could be a potentitial biological scaffold on tissue engineering application.