Purification of a Pegylated Protein

PEGylation can bring along many challenges, one of which is the formation of process related impurities. Typically, size exclusion chromatography has been advantageous in removal of these impurities at small-scale, but this is not feasible for large-scale implementation. With the use of High Throughput Screens (HTS), several other purification methods were identified. This poster will highlight a challenging process in which large polymer-protein complexes were conjugated and subsequently purified downstream using HIC chromatography.