Transcriptome Analysis of Pyropia Yezonesis Compared with Heat-Stress Tolerance Mutant Using RNA-Seq

Pyropia yezonesis is one of the macro marine algae and is used to sea crop in the East Asia such as Korea, China and Japan. P. yezonesis is present mostly in intertidal zone. Due to the feature of the growth environment, it is received many kinds of stress during growing such as light, temperature, nutrient and dry. It is important to withstand these stress conditions for growing. In recently, many studies are tried to selecting pyropia strain had stress-tolerance. But, there are very few studies understanding molecular mechanisms of stress-tolerance mechanisms in pyropia.

So, we made the heat-stress tolerance P. yezonesis mutant stain using radiation and got whole transcriptomes using Next generation RNA Sequencing (RNA-seq) to study stress-tolerance mechanisms. RNA-seq reads were assembled using Trinity with de novo assembly mode and generated 31,147 and 78,793 unigenes for mutant and WT, respectively. For downstream analysis, the two transcriptomes were annotated by searching homologous sequences in NCBI NR, Swissprot, Pfam, and KEGG database. We analyzed differentially expressed unigenes and gene ontology (GO) enrichment analysis using shared sequences between WT and mutant. And putative Single Nucleotide Polymorphisms (SNPs) were discovered between two transcriptome sets (shared or unshared).