Abstract: Nano-Constructs Fabricated From a Genome-Depleted Plant Virus and Indocyanine Green for near Infrared Imaging and Phototherapy (SBE’s 6th International Conference on Bioengineering and Nanotechnology)

Bahman Anvari¹, Yadir Guerrero¹, Bongsu Jung¹, Baharak Bahmani¹, Ayala L. N. Rao², Sheela Singh³, Valentine I. Vullev¹ and Vikas Kundra³, (1)Bioengineering, University of California, Riverside, Riverside, CA, (2)Plant Pathology and Microbiology, University of California, Riverside, Riverside, CA, (3)Diagnostic Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX

We have constructed a new type of an optical nano-material composed of genome-depleted plant infecting brome mosaic virus (BMV) doped with FDA-approved near infrared (NIR) chromophore, indocyanine green (ICG). We refer to these constructs as optical viral ghosts (OVGs) since only the capsid protein (CP) subunits of the BMV remain to encapsulate ICG. The abundance of naturally present amines on the CP shell of the OVGs provide addressable sites for covalent attachment of targeting moieties such as antibodies using a simple method based on reductive amination. OVGs can serve as a theranostic construct since the same chromophore (ICG) may be used for both imaging and phototherapy. One of our areas of interest for biomedical applications of OVGs is in their potential use for real-time intraoperative NIR fluorescence imaging and photodestruction of small peritoneal ovarian tumor nodules. In this presentation, we will provide results associated with physical characterization of OVGs, and their ability to target and image ovarian cancer cells in-vitro.

Acknowledgement: NSF grant CBET-1144237.

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See more of this Session: Session 2: Nanoparticles, Nanocomposites and Nanoporous Materials for Bio-Applications
See more of this Group/Topical: 6th International Conference on Bioengineering and Nanotechnology