Use of Electrodialysis with Ultrafiltration Membrane for the Simultaneous Enzymatic Hydrolysis Production and Fractionation of Bioactive Peptides From Beta -Lactoglobulin

The hydrolysis of β-lactoglobulin protein and the fractionation of generated peptides were performed in one step in an EDUF cell. After 240 min of treatment, amongst the 30 peptides peaks detected in the β-lactoglobulin hydrolysate which corresponded to 30 peptides, respectively, 16 and 4 peptides were detected in the anionic and cationic peptide recovery compartment. Amongst these 16 peptides, 2 hypocholesterolemic peptides, 3 antihypertensive peptides and 1 antibacterial peptide were recovered and concentrated with migration rates ranged between 5.5 and 66.0%. Amongst the 4 cationic peptides, the peptide sequence ALPMHIR, identified as lactokinin and known to exert an important antihypertensive effect, was recovered and concentrated. At our knowledge, it was the first time that hydrolysis was conducted under an electric field to simultaneously separate anionic and cationic peptides produced.