Incorporation of Long-Read Technologies into Hybrid-de novo Genome Sequencing Strategy: An Assessment on PacBio and Moleculo Read

De novo sequencing of large genomes using NGS technology generally requires combination of resources for the production of raw reads and for the genomic assembly depending on the characteristics of your target genomes. Frequently used DNA libraries for this purpose include short paired-end libraries roughly ranging from 300 bp to-800 bp, and mate-pair libraries of 2-3 kb to 35kb. Development of SMRT technology and PACBIO RS analyzer (Pacific Biosciences of California, Inc.) produces strand-specific long reads up to 10 kb range, and added new strategy for de novo assembly. Similarly, more recently developed Moleculo technology by Illumina, Inc. provides us additional resources for strand-specific long reads of 8-10 kb size range. In this meeting, we will report on the characteristics and comparison of the sequence reads produced from these technologies using published and in-house assemblies on the genome of Monosiga ovate, a fresh-water uni-cellular choanoflagellate, which is regarded as the extant relatives of the metazoan, and the genome of the marine species, Monosiga brevicollis is already published (Nature 451, 2008). We also sequenced genome of Branchiostoma belcheri, which was captured at the Pacific coast of Japan. Lancelets belongs to cephalochordate and is now regarded as the closest ancestor of vertebrates. Genome sequence of the Atlantic strain, Branchiostoma floridae, was also published (Nature 453, 2008).