P0080
Analyses of Long Non-Coding RNA using RNA Sequencing during the Pre-Implantation Phase in Pig Endometrium
Establishment of implantation in pig is accompanied by a coordinated interaction between the maternal uterine endometrium and developing conceptus. Previous transcriptome studies mainly focused on protein-coding transcripts of gene expression and regulatory networks in pre-implantation. Recently, long non-coding RNA (LncRNA) has been a new horizon in understanding gene expression.Thus, we performaed a whole transcriptome analysis of lncRNA and mRNA to clarify the mechanisms of pre-implantation in pig endometrium.
Results:
In this study, we investigated differences in the expression profiles of endometrial tissue on days 9, 12 and 15 of pregnancy and on day 12 of non-pregnancy in Yorkshire, and described a comprehensive analysis of lncRNAs in endometrial tissue samples by using RNA-seq. 2805 novel lncRNAs, 2,376 (301 lncRNA and 2075 mRNA) differentially expressed genes (DEGs) and 2149 novel transcripts were obtained by pairwise comparison. In agreement with the previously reports, lncRNAs shared similar characteristics, such as shorter, lower in exon number, lower in expression level and less conserved than protein coding transcripts. Bioinformatics analysis showed that the DGEs were involved in protein binding, cellular process, immune system process and enriched in focal adhesion, Jak-STAT, FoxO and MAPK signaling pathway. We also found that two lncRNAs TCONS_01729386 and TCONS_01325501 played a vital role in embryo pre-implantation. Further, the expression of FGF7, NMB, COL5A3, S100A8 and PPP1R3D genes were significantly upregulated at the time of maternal recognition of pregnancy.
Conclusions:
Our results first identified the characterization and expression profile of lncRNAs in pig endometrium during pre-implantation phase and provided a resource for lncRNA studies in other noninvasive animal implantation.