Breed Discrimination Analysis in South Asian Duck Breeds using Fourteen Microsatellite Markers

Native duck breeds are important genetic resources which are used for valuable protein source and healthy food in Asian countries. Recently, conservation of native ducks has been performed, mainly for the development of new synthetic breeds. In this point of view, the genetic markers for breed discrimination can be a useful tool for breeding strategies and prevent to fraud in the market. Therefore, this study initially investigated 100 microsatellite (MS) markers, which were tested for discrimination analysis using South Asian duck breeds and commercial ducks. As the result, we selected 14 highly polymorphic markers in South Asian duck breeds. The average number of alleles, expected heterozygosity (Hexp) value and polymorphic information contents (PIC) were calculated at 12.07, 0.769 and 0.736, for these markers, respectively. The expected probability of identity (PI) value in random individuals, random half-sibs and random sibs values were 7.46E-17, 1.40E-12 and 1.47E-06, respectively. The commercial ducks can be discriminated well with South Asian duck breeds but some of Korean native ducks have shared haplotypes with commercial duck and Bangladesh native duck breeds. These MS markers combination can provide useful information for discriminating of duck breeds with further verification.

Key words: South Asian duck, microsatellite marker, genetic marker, discrimination, Korean native duck