Genetic Structure and Diversity of Southeast Asian Native Pig Populations Analyzed Using SNPs

�[Introduction-Sus scrofa has a very wide natural distribution, therefore the origin of domestic pig has long been an object of discussion. Thus far, phylogenetic analyses have been conducted using polymorphisms of serum protein, mitochondriaDNA and microsatellites. There was an enormous number of SNPs in porcine genome, and if they are analyzed as DNA markers, it enable us to conduct accurate phylogenetic analyses.In order to reveal genetic relationships among Asian wild boars or pigs including Southeast Asia native pigs, Ryukyu wild boars (RWBs) and Japanese wild boars (JWB), 96 SNPs were genotyped, and phylogenetic analysis was carried out.

�[Materials and methods-Southeast native pigs tissues (n=132) were collected from 5 countries, Vietnam, Lao, Cambodia, Myanmar and Bhutan. In addition, JWB and RWB were tested. The96 SNPs were genotyped using DigiTag2 assay (Nishida et al., 2007). Phylogenetic analysis (Neighbor-Joining method; NJ), and STRUCTURE were conducted.

�[Result and discussion-The NJ tree tended to classify these samples into 6 clades. Southeast native pigs and wild boars were classified into 4 clades: pigs living along west side of Mekong, Mong Cai and I pig, pigs living along east side of Mekong and Cambodia and Bhutan boars. RWBs were not genetically close to Southeast native pigs and Wild Boars. According to previous study, RWBs were genetically close to Vietnamese Pigs (Hongo et al., 2002). This result suggested that there were genetic distances among RWBs, and Southeast native pigs and Wild Boars had various genetic structures.