Cotton bollworm (Helicoverpa armigera) is one of damaging pests for soybean (Glycine max). Identification and utilization of native resistance genes in soybean are one of economical and environmentally means to control this pest. A wild soybean (Glycine soja), ED059, was highly resistant to cotton bollworm in comparison with cv. Tianlong 2, PI535807 and PI 533604 in either choice or nochoice assays. In order to reveal the resistance mechanism, we conducted transcript and protein profiling for ED059 and a susceptible cultivar, 05-4, at 24h after treatment with cotton bollworm. The results showed that 2213 transcripts and 116 proteins were specifically regulated in ED059, which included protein kinases, transcriptional factors and genes involved in hormone biosynthesis and signal transduction. Noticeable, a large number of genes related to antinutritive or toxic proteins were specifically induced in ED059. Function of a potential resistant gene was confirmed by transgenic studies in Arabidopsis. Currently, QTL mapping of resistance gene in ED059 were ongoing in our lab.
