Databases of Plant Genome Sequences and DNA Markers in Kazusa DNA Research Institute

Hideki Hirakawa , Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
Hideki Nagasaki , Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
Akihiro Nakaya , Osaka University, Suita, Osaka, Japan
Hisako Ichihara , Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
Erika Asamizu , Ryukoku University, Otsu, Shiga, Japan
Sachiko Shirasawa , Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
Taeko Shibaya , Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
Yasukazu Nakamura , Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
Kenta Shirasawa , Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
Sachiko Isobe , Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
Satoshi Tabata , Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
Since the release of the whole genome sequence of Arabidopsis thaliana in 2000, we have determined the structures of the genomes of various model plants and crops which have a wide range of genome sizes and different ploidy using both Sanger sequencing and next-generation sequencing (NGS) technologies, and the obtained data have been released through public DNA databases and the individual genome databases from Kazusa DNA Research Institute (KDRI) (http://www.kazusa.or.jp/e/resources/database.html). The target plants include Solanum lycopersicum (tomato; estimated genome size: 900 Mb, polyploidy: diploid), Jatropha curcas (jatropha; 410 Mb, diploid), Eucalyptus camaldulensis (river red gum; 650 Mb, diploid), Solanum melongena (eggplant; 1.1 Gb, diploid), Raphanus sativus (radish; 530 Mb, diploid), Ipomoea trifida (wild sweetpotato; 520 Mb, diploid), and Fragaria × ananassa (strawberry; 800 Mb, octoploid). In addition, polymorphism analyses have been conducted for several crops, and the DNA markers developed for 13 plant species have been released from Kazusa Marker DataBase (http://marker.kazusa.or.jp). Recently, we generated a comprehensive genome database for plants, Plant Genome DataBase Japan (PGDBj; http://pgdbj.jp/?ln=en), which enables cross-searches on the following sub-databases: an orthologous database for plants and cyanobacteria (Ortholog DB), a plant resource database (Plant Resource DB), a DNA marker database for 80 plant species (DNA marker DB). In this presentation, the latest status of the above databases will be introduced.