The Assembly and Analysis of Wheat Chromosome 7A

The assembly of the approximately 800Mb bread wheat chromosome 7A will be presented, including updates on the integration of mate-pair data and Bionano maps.

Particular challenges in the production of the whole-chromosome pseudomolecule will be discussed. In particular, an integrated approach for identifying and filtering cross-BAC-pool contamination, utilising mate-pair data, Bionano maps and the original BAC fingerprints will be described. The analysis of the low recombination region which includes the centromere, provides a focus for this presentation since it appears to have additional distinctive features such as containing most of the constitutively expressed genes.