Functional Genomics Using the Genome and Transcriptome of Nicotiana benthamiana

Hyungtaek Jung , Queensland University of Technology, Brisbane, Australia
Nicotiana benthamiana is widely used as a model plant for functional genomics, synthetic metabolic engineering, RNA interference and plant-microbe interaction research. An openly accessible interactive website (www.benthgenome.qut.edu.au) has been set up to facilitate examination of the laboratory (LAB), a popular GFP-expressing line (16C) and wild strain genomes and transcriptomes, their gene expression patterns, their microRNA regulation and their CRISPR-Cas gene editing. We highlight the comparative assembly statistics from de novo and reference-guided assembly approaches, and insights about the variability of the species caused by a 72nt insertion in RDR1 gene (LAB, an accession from South Australia).