W423
Cloning of a Broad Spectrum Stem Rust Resistance Gene in Wheat

Date: Saturday, January 12, 2013
Time: 11:35 AM
Room: Town and Country
Sambasivam Periyannan , CSIRO Plant Industry, Canberra, Australia
John Moore , CSIRO Plant Industry, Canberra, Australia
Harbans Bariana , University of Sydney, Narellan, Australia
Karin R. Deal , University of California, Davis, Davis, CA
Xiaojing Wang , Montana State University, Bozeman, MT
Mingcheng Luo , University of California, Davis, CA
Li Huang , Montana State University, Bozeman, MT
Michael Ayliffe , CSIRO, Canberra, Australia
Urmil Bansal , University of Sydney, Narellan, Australia
Xiuying Kong , Chinese Academy of Agricultural Sciences, Beijing, China
Peter Dodds , CSIRO Plant Industry, Canberra, Australia
Jan Dvorak , University of California, Davis, Davis, CA
Evans Lagudah , CSIRO Plant Industry, Canberra, Australia
PDF file
Stem rust resistance gene Sr33 derived from Aegilops tauschii and transferred into bread wheat (Triticum aestivum) is effective against all stem rust pathogen (Puccinia graminis) derivatives of Ug99 and known wheat stem rust races prevalent in Australia, Canada, Ethiopia, India, United States, South Africa and South America.  A combination of high resolution genetic and physical mapping was used to define the Sr33 locus. Sequence analysis of BAC contig spanning the Sr33 locus revealed the presence of a cluster of CC-NBS-LRR genes. EMS mutants that inactivate Sr33 resistance were used to identify the gene member responsible for stem rust resistance function. Further confirmation of the candidate Sr33 gene was obtained using barley stripe mosaic virus induced gene silencing. Characterization of Sr33-mediated stem rust resistance indicated that Sr33 protein function is independent of RAR1, SGT1 and HSP90 chaperones and uncoupled from direct protein-protein interaction with selective members of WRKY transcription factor family.
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