Evaluation of Chloroplast Genotypes of Korean Cucumber Cultivars (Cucumis sativus L.) Using sdCAPS Markers Related to Chilling Tolerance

DNA marker can determine the genotype of many species. Single nucleotide polymorphism (SNP) detection is difficult without sequencing but it becomes easier with sdCAPS methods. Here an experiment was performed for developing molecular markers using two SNPs, CSatpB-SNP and CSycf1-SNP of chloroplast in cucumber plants. Properly designed primers with restriction enzyme sites proved success of PCR and efficacy of restriction enzymes itself. These markers then used to study the genotyping behavior of cucumber breeding lines and cultivars obtained from various sources in respect of their chilling stress response. ‘NC76’ and ‘Chipper’ showed genotypes related to chilling tolerance while others including all Korean cucumber cultivars look genotypes related to chilling susceptible. Therefore our data indicates that both maternal and a nuclear factors related to chilling tolerance can be transferred from ‘NC76’ to Korean cucumber cultivars when ‘NC76’ used as a mother source in crossing