Whole Genome Mapping of 3-Dimensional Root System Architecture in Rice (Oryza sativa L.)

Plant root systems and associated symbiotic organisms act as critical links between the growing shoot and the rhizosphere, providing both vital nutrients and water to sustain growth.  The efficient quantification of root traits remains a key bottleneck in effectively utilizing genomic resources during the study of root system development and function.  For our research, a high-throughput 3-dimensional (3D) root phenotyping platform was developed to take advantage of publicly available germplasm and genotypic information.  The phenotyping platform allows whole root system architecture (RSA) characteristics of growing seedlings to be measured and tracked.  Using our phenotyping tools in combination with public genetic resources, we are beginning to identify hotspot regions of the rice (Oryza sativa) genome that are involved in root system development through joint linkage-association mapping studies.  These genomic regions are being further explored for candidate genes as well as possible functional roles in root system adaption to abiotic stresses such as water or nutrient limitations.