Senjuti Sinharoy
,
The Samuel Roberts Noble Foundation, Ardmore, OK
Igor Kryvoruchko
,
The Samuel Roberts Noble Foundation, Ardmore, OK
Ivone Torres-Jerez
,
The Samuel Roberts Noble Foundation, Ardmore, OK
Jiangqi Wen
,
The Samuel Roberts Noble Foundation, Ardmore, OK
Mingyi Wang
,
The Samuel Roberts Noble Foundation, Ardmore, OK
Nick Krom
,
The Samuel Roberts Noble Foundation, Ardmore, OK
Xiaofei Cheng
,
The Samuel Roberts Noble Foundation, Ardmore, OK
Jin Nakashima
,
The Samuel Roberts Noble Foundation, Ardmore, OK
Shulan Zhang
,
The Samuel Roberts Noble Foundation, Ardmore, OK
Mark Taylor
,
The Samuel Roberts Noble Foundation, Ardmore, OK
Yuhong Tang
,
The Samuel Roberts Noble Foundation, Ardmore, OK
Elison Blancaflor
,
The Samuel Roberts Noble Foundation, Ardmore, OK
Rujin Chen
,
The Samuel Roberts Noble Foundation, Ardmore, OK
Kiran S. Mysore
,
The Samuel Roberts Noble Foundation, Ardmore, OK
Vagner Benedito
,
West Virginia University, Morgantown, WV
Jeremy Murray
,
John Innes Centre, Norwich, United Kingdom
Giles Oldroyd
,
John Innes Centre, Norwich, United Kingdom
Pascal Ratet
,
ISV-CNRS, Gif-sur-Yvette, France
Michael K. Udvardi
,
The Samuel Roberts Noble Foundation, Ardmore, OK
In recent years numerous genetic resources have been developed for the model legume
Medicago truncatula, including
Tnt1-insertion and fast-neutron deletion mutant populations, the Gene Expression Atlas (MtGEA), and the largely complete genome sequence. Transcriptomic studies revealed that thousands of plant genes are involved in SNF; however, a small fraction of them has been functionally characterized.
From a tobacco retrotransposon Tnt1-insertion mutant population of M. truncatula ecotype R108 we isolated 179 mutants impaired in nodule development and SNF during the interaction with Sinorhizobium meliloti. In this collection we identified 39 alleles of known symbiotic genes. With many potentially new symbiotic mutants, this collection is a valuable resource for cloning novel symbiotic genes via Tnt1 tagging. Some of these mutants will be described in detail. The phenotype in one of the Fix- mutants co-segregates with Tnt1 insertion in a gene annotated as “embryo-specific 3”, a member of the PLAT (Polycystin-1; Lipoxygenase, Alpha-Toxin domain or LH2 (Lipoxygenase Homology 2) superfamily, which was named the Nodulation-specific PLAT-domain gene, NPD1, and will be presented in more detail.
To expand MtGEA, a spatially-resolved gene expression inventory in Medicago nodules was generated. This provides unprecedented resolution, for linking nodule zone-specific gene expression with molecular processes necessary for nodule development and SNF. This fine-tuned expression inventory is being used to target genes of interest for functional characterization via reverse-genetics. Tnt1-insertion mutants in a transcription factor (TF), a C2H2 family member, and a MATE5 transporter of the multidrug and toxin efflux family will be presented.
