TrueSight: A New Algorithm For Splice Junction Detection Using RNA-Seq

RNA-seq has proven to be a powerful technique for transcriptome profiling based on next-generation sequencing (NGS) technologies. However, due to the short length of NGS reads, it is challenging to accurately map RNA-seq reads to splice junctions (SJs), which is a critically important step in the analysis of alternative splicing (AS) and isoform construction. In this talk, I will introduce a new method that we recently developed, called TrueSight. The method uses a machine-learning approach to precisely identify SJs. Both simulations and real data evaluations showed that TrueSight achieved higher sensitivity and specificity than other methods. We believe that TrueSight will be highly useful to comprehensively study the biology of alternative splicing.