Microspore Culture of Brassica rapa

19 plant materials of Brassica rapa used for microspore culture sent from the Wageningen university of Netherlands. Nineteen plant materials were total 8 subspecies contained Chinese cabbage, Chinese turnip cabbage, Pak choi, Turnip, Broccolleto, Mizuna, Komatsuna and Turnip green. In all plant materials examined, embryos were obtained from 13 plant materials, these were 6 types except Komatsuna and Turnip Green. The embryo yields were significantly different between these 13 plant materials, and we expect embryo development of 17 plant materials using more various culture condition. We were regenerated normal plants from 10 plant materials that survived more than 4 weeks in the soil using microspore culture. Nine plant materials were induced bolting after 4 weeks vernalization at 5℃ and harvested seeds from 5 plant materials. In this case of Mizuna, we obtained 3791 seeds from one plant of normal growth and total 7318 seeds were harvested from 5 plant materials of 4 types(Chinese cabbage, Chinese turnip cabbage, Pak choi, Mizuna). At present, we carry out experiment for obtain more seeds and induce embryos from the other plant materials.